Recovery of microquantities of human Epidermal Growth Factor from Esherichia coli homogenate and Pichia pastoris culture medium using expanded bed adsorption

A rational design of recovery of microquantities of human Epidermal Growth Factor (hEGF) from different complex feedstocks using STREAMLINE Direct HST in expanded bed adsorption (EBA) was approached. The highest adsorption yields were achieved at pH 4.5, which was close to the isoelectric point of p...

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Main Authors: Rosti, Ira Amira, Ramanan, Ramakrishnan Nagasundara, Tan, Joo Shun, Ling, Tau Chuan, Ariff, Arbakariya
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出版: Taylor & Francis Inc. 2014