β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells
β-Mangostin (βM) was isolated from Cratoxylum arborescens to investigate its anti-cancer effect in MCF-7 cells. βM induced apoptosis by down-regulation of Bcl2 and up-regulation of Bax, triggering the cytochrome c release from mitochondria to cytosol. The release of caspase-9 and -7 and consequently...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36671/1/%CE%B2.pdf |
| _version_ | 1796972959832735744 |
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| author | Syam, Suvitha Bustamam, Ahmad Abdullah, Rasedee Sukari, Mohd Aspollah Mohd Hashim, Najihah Ghaderian, Mostafa Rahmani, Mawardi Mohan, Syam Abdelwahab, Siddig Ibrahim Mohd Ali, Hapipah |
| author_facet | Syam, Suvitha Bustamam, Ahmad Abdullah, Rasedee Sukari, Mohd Aspollah Mohd Hashim, Najihah Ghaderian, Mostafa Rahmani, Mawardi Mohan, Syam Abdelwahab, Siddig Ibrahim Mohd Ali, Hapipah |
| author_sort | Syam, Suvitha |
| collection | UPM |
| description | β-Mangostin (βM) was isolated from Cratoxylum arborescens to investigate its anti-cancer effect in MCF-7 cells. βM induced apoptosis by down-regulation of Bcl2 and up-regulation of Bax, triggering the cytochrome c release from mitochondria to cytosol. The release of caspase-9 and -7 and consequently cleaved PARP leading to apoptotic was observed upon treatment. Reduction of both bid and caspase 8 and the up regulation of Fas showed the involvement of the extrinsic pathway. Significantly up regulated GADD45A and HRK genes were observed upon treatment, with concomitant inhibition of NF-kB to nucleus. The protein array had demonstrated the expression of HSP 70, HSP 60, XIAP, Survivin, p53 and Bax. Moreover, βM had showed p53-dependent G2/M cell cycle arrest by down regulation of cdc2 and PCNA. Together, the results demonstrated that the βM induced anti-proliferative effect, leading to G2/M phase cell cycle arrest and apoptosis through both the extrinsic and mitochondrial pathways with the involvement of the multiple pro and anti-apoptosis and NF-kB signalling pathways. |
| first_indexed | 2024-03-06T08:36:01Z |
| format | Article |
| id | upm.eprints-36671 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:36:01Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-366712016-01-26T07:23:11Z http://psasir.upm.edu.my/id/eprint/36671/ β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells Syam, Suvitha Bustamam, Ahmad Abdullah, Rasedee Sukari, Mohd Aspollah Mohd Hashim, Najihah Ghaderian, Mostafa Rahmani, Mawardi Mohan, Syam Abdelwahab, Siddig Ibrahim Mohd Ali, Hapipah β-Mangostin (βM) was isolated from Cratoxylum arborescens to investigate its anti-cancer effect in MCF-7 cells. βM induced apoptosis by down-regulation of Bcl2 and up-regulation of Bax, triggering the cytochrome c release from mitochondria to cytosol. The release of caspase-9 and -7 and consequently cleaved PARP leading to apoptotic was observed upon treatment. Reduction of both bid and caspase 8 and the up regulation of Fas showed the involvement of the extrinsic pathway. Significantly up regulated GADD45A and HRK genes were observed upon treatment, with concomitant inhibition of NF-kB to nucleus. The protein array had demonstrated the expression of HSP 70, HSP 60, XIAP, Survivin, p53 and Bax. Moreover, βM had showed p53-dependent G2/M cell cycle arrest by down regulation of cdc2 and PCNA. Together, the results demonstrated that the βM induced anti-proliferative effect, leading to G2/M phase cell cycle arrest and apoptosis through both the extrinsic and mitochondrial pathways with the involvement of the multiple pro and anti-apoptosis and NF-kB signalling pathways. Elsevier 2014-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36671/1/%CE%B2.pdf Syam, Suvitha and Bustamam, Ahmad and Abdullah, Rasedee and Sukari, Mohd Aspollah and Mohd Hashim, Najihah and Ghaderian, Mostafa and Rahmani, Mawardi and Mohan, Syam and Abdelwahab, Siddig Ibrahim and Mohd Ali, Hapipah (2014) β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells. Journal of Functional Foods, 6. pp. 290-304. ISSN 1756-4646; ESSN: 2214-9414 10.1016/j.jff.2013.10.018 |
| spellingShingle | Syam, Suvitha Bustamam, Ahmad Abdullah, Rasedee Sukari, Mohd Aspollah Mohd Hashim, Najihah Ghaderian, Mostafa Rahmani, Mawardi Mohan, Syam Abdelwahab, Siddig Ibrahim Mohd Ali, Hapipah β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells |
| title | β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells |
| title_full | β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells |
| title_fullStr | β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells |
| title_full_unstemmed | β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells |
| title_short | β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells |
| title_sort | β mangostin induces p53 dependent g2 m cell cycle arrest and apoptosis through ros mediated mitochondrial pathway and nfkb suppression in mcf 7 cells |
| url | http://psasir.upm.edu.my/id/eprint/36671/1/%CE%B2.pdf |
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