Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essentia...
| Auteurs principaux: | , , , |
|---|---|
| Format: | Article |
| Langue: | English |
| Publié: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2012
|
| Accès en ligne: | http://psasir.upm.edu.my/id/eprint/41054/1/iochemical%20properties%20of%20red%20tilapia%20%28Oreochromis%20niloticus%29%20protein%20hydrolysates.pdf |
| _version_ | 1825949713113808896 |
|---|---|
| author | Shamloo, Maryam Bakar, Jamilah Mat Hashim, Dzulkifly Khatib, Alfi |
| author_facet | Shamloo, Maryam Bakar, Jamilah Mat Hashim, Dzulkifly Khatib, Alfi |
| author_sort | Shamloo, Maryam |
| collection | UPM |
| description | The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essential amino acids to non-essential amino acids increased after hydrolysis in all samples; however, no significant differences among them were observed. AH had a highest (P < 0.05) DPPH radical-scavenging activity, but no significant difference in the DPPH between FH and PH was observed. SDS-PAGE patterns for all the hydrolysates showed significant (P < 0.05) reduction in the number and the intensity of the bands with increasing time of hydrolysis. Flavourzyme showed the lowest rate of hydrolytic activity towards the tilapia mince. |
| first_indexed | 2024-03-06T08:48:47Z |
| format | Article |
| id | upm.eprints-41054 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:48:47Z |
| publishDate | 2012 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-410542015-12-10T00:51:10Z http://psasir.upm.edu.my/id/eprint/41054/ Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates Shamloo, Maryam Bakar, Jamilah Mat Hashim, Dzulkifly Khatib, Alfi The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essential amino acids to non-essential amino acids increased after hydrolysis in all samples; however, no significant differences among them were observed. AH had a highest (P < 0.05) DPPH radical-scavenging activity, but no significant difference in the DPPH between FH and PH was observed. SDS-PAGE patterns for all the hydrolysates showed significant (P < 0.05) reduction in the number and the intensity of the bands with increasing time of hydrolysis. Flavourzyme showed the lowest rate of hydrolytic activity towards the tilapia mince. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41054/1/iochemical%20properties%20of%20red%20tilapia%20%28Oreochromis%20niloticus%29%20protein%20hydrolysates.pdf Shamloo, Maryam and Bakar, Jamilah and Mat Hashim, Dzulkifly and Khatib, Alfi (2012) Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates. International Food Research Journal, 19 (1). pp. 183-188. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/19%20%2801%29%202011/%2824%29IFRJ-2011-144%20Jamilah.pdf |
| spellingShingle | Shamloo, Maryam Bakar, Jamilah Mat Hashim, Dzulkifly Khatib, Alfi Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_full | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_fullStr | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_full_unstemmed | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_short | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_sort | biochemical properties of red tilapia oreochromis niloticus protein hydrolysates |
| url | http://psasir.upm.edu.my/id/eprint/41054/1/iochemical%20properties%20of%20red%20tilapia%20%28Oreochromis%20niloticus%29%20protein%20hydrolysates.pdf |
| work_keys_str_mv | AT shamloomaryam biochemicalpropertiesofredtilapiaoreochromisniloticusproteinhydrolysates AT bakarjamilah biochemicalpropertiesofredtilapiaoreochromisniloticusproteinhydrolysates AT mathashimdzulkifly biochemicalpropertiesofredtilapiaoreochromisniloticusproteinhydrolysates AT khatibalfi biochemicalpropertiesofredtilapiaoreochromisniloticusproteinhydrolysates |