Heat and hydrolytic enzymes treatment improved the Agrobacterium-mediated transformation of recalcitrant indica rice (Oryza sativa L.)

Reported Agrobacterium transformation efficiency of indica rice shoot apices varied from 0.4 to 13.8 %. In order to improve the transformation efficiency, modification of transformation protocols through heat and hydrolytic enzyme treatments on rice shoot apices were carried out. Transient expressio...

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Bibliographic Details
Main Authors: Wong, Clement Kiing Fook, Lai, Kok Song, Wong, Mui Yun, Mahmood, Maziah
Format: Article
Language:English
Published: Springer 2016
Online Access:http://psasir.upm.edu.my/id/eprint/43352/1/Heat%20and%20hydrolytic%20enzymes%20treatment%20improved%20the%20Agrobacterium-mediated%20transformation%20of%20recalcitrant%20indica%20rice%20%28Oryza%20sativa%20L.%29.pdf