Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations
This study compares the distinctive performance, yield and characteristics of bioflocculants (UPMBF13) derived from submerged (SmF) and solid-state fermentation (SSF) of Bacillus subtilis UPMB13. The bioflocculants were subjected to morphological characterization, functional groups determination, m...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mary Ann Liebert
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/45818/1/BACILLUS.pdf |
| _version_ | 1796974579605831680 |
|---|---|
| author | Zulkeflee, Zufarzaana Yusoff, Mohd Kamil Aris, Ahmad Zaharin Shamsuddin, Zulkifli Sánchez, Antoni Komilis, Dimitrios |
| author_facet | Zulkeflee, Zufarzaana Yusoff, Mohd Kamil Aris, Ahmad Zaharin Shamsuddin, Zulkifli Sánchez, Antoni Komilis, Dimitrios |
| author_sort | Zulkeflee, Zufarzaana |
| collection | UPM |
| description | This study compares the distinctive performance, yield and characteristics of bioflocculants (UPMBF13) derived from submerged (SmF) and solid-state fermentation (SSF) of Bacillus subtilis UPMB13. The bioflocculants were subjected to morphological characterization, functional groups determination, molecular weight measurement, protein content quantification and specific compound identification. The SmF and the SSF strategies yielded an average of 2.70 g l -1 and 1.25 g kg-1 of UPMBF13, respectively. The submerged UPMBF13 resulted in a clear suspension with visible flocs formation, while flocs from the SSF treatment were less apparent. Higher total proteinaceous contents of UPMBF13 from the SSF led to the inferiority in flocculating performances. The produced UPMBF13 consisted of hydroxyl, carboxyl, methoxyl and carbonyl functional groups which contributed to their flocculating abilities. The molecular weights of UPMBF13 were around 10-50 kDa, characterizing them into low-molecular weight bioflocculants. The submerged UPMBF13 were more fibrous in nature than the SSF, giving the submerged
UPMBF13 the upper hand in flocculation. SmF was the best fermentation method yielding high performing bioflocculants at a faster rate by the utilization of non-elaborative techniques. SSF, on the other hand, was proven feasible but further improvements are needed. |
| first_indexed | 2024-03-06T08:59:06Z |
| format | Article |
| id | upm.eprints-45818 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:59:06Z |
| publishDate | 2015 |
| publisher | Mary Ann Liebert |
| record_format | dspace |
| spelling | upm.eprints-458182021-01-18T18:51:57Z http://psasir.upm.edu.my/id/eprint/45818/ Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations Zulkeflee, Zufarzaana Yusoff, Mohd Kamil Aris, Ahmad Zaharin Shamsuddin, Zulkifli Sánchez, Antoni Komilis, Dimitrios This study compares the distinctive performance, yield and characteristics of bioflocculants (UPMBF13) derived from submerged (SmF) and solid-state fermentation (SSF) of Bacillus subtilis UPMB13. The bioflocculants were subjected to morphological characterization, functional groups determination, molecular weight measurement, protein content quantification and specific compound identification. The SmF and the SSF strategies yielded an average of 2.70 g l -1 and 1.25 g kg-1 of UPMBF13, respectively. The submerged UPMBF13 resulted in a clear suspension with visible flocs formation, while flocs from the SSF treatment were less apparent. Higher total proteinaceous contents of UPMBF13 from the SSF led to the inferiority in flocculating performances. The produced UPMBF13 consisted of hydroxyl, carboxyl, methoxyl and carbonyl functional groups which contributed to their flocculating abilities. The molecular weights of UPMBF13 were around 10-50 kDa, characterizing them into low-molecular weight bioflocculants. The submerged UPMBF13 were more fibrous in nature than the SSF, giving the submerged UPMBF13 the upper hand in flocculation. SmF was the best fermentation method yielding high performing bioflocculants at a faster rate by the utilization of non-elaborative techniques. SSF, on the other hand, was proven feasible but further improvements are needed. Mary Ann Liebert 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45818/1/BACILLUS.pdf Zulkeflee, Zufarzaana and Yusoff, Mohd Kamil and Aris, Ahmad Zaharin and Shamsuddin, Zulkifli and Sánchez, Antoni and Komilis, Dimitrios (2015) Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations. Environmental Engineering Science, 18. pp. 1-10. ISSN 1092-8758 https://journal.gnest.org/sites/default/files/Submissions/gnest_01949/gnest_01949_proof.pdf |
| spellingShingle | Zulkeflee, Zufarzaana Yusoff, Mohd Kamil Aris, Ahmad Zaharin Shamsuddin, Zulkifli Sánchez, Antoni Komilis, Dimitrios Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations |
| title | Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations |
| title_full | Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations |
| title_fullStr | Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations |
| title_full_unstemmed | Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations |
| title_short | Performance, yield and characteristics of bioflocculants (UPMB13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentations |
| title_sort | performance yield and characteristics of bioflocculants upmb13 produced by bacillus subtilis upmb13 during submerged and solid state fermentations |
| url | http://psasir.upm.edu.my/id/eprint/45818/1/BACILLUS.pdf |
| work_keys_str_mv | AT zulkefleezufarzaana performanceyieldandcharacteristicsofbioflocculantsupmb13producedbybacillussubtilisupmb13duringsubmergedandsolidstatefermentations AT yusoffmohdkamil performanceyieldandcharacteristicsofbioflocculantsupmb13producedbybacillussubtilisupmb13duringsubmergedandsolidstatefermentations AT arisahmadzaharin performanceyieldandcharacteristicsofbioflocculantsupmb13producedbybacillussubtilisupmb13duringsubmergedandsolidstatefermentations AT shamsuddinzulkifli performanceyieldandcharacteristicsofbioflocculantsupmb13producedbybacillussubtilisupmb13duringsubmergedandsolidstatefermentations AT sanchezantoni performanceyieldandcharacteristicsofbioflocculantsupmb13producedbybacillussubtilisupmb13duringsubmergedandsolidstatefermentations AT komilisdimitrios performanceyieldandcharacteristicsofbioflocculantsupmb13producedbybacillussubtilisupmb13duringsubmergedandsolidstatefermentations |