Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines
Oestrogens play an important role in the development of breast cancer. Oestrone sulphate (E1S) acts as a huge reservoir of oestrogens in the breast and is converted to oestrone (E1) by oestrone sulphatase (E1STS). E1 is then reversibly converted to the potent oestrogen, oestradiol (E2) by oestradiol...
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| Format: | Article |
| Language: | English |
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Royal College of Pathologists of Australasia
2001
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| Online Access: | http://psasir.upm.edu.my/id/eprint/50002/1/Effect%20of%20transforming%20growth%20factor-%CE%B21%2C%20insulin-like%20growth%20factor-I%20and%20insulin-like%20growth%20factor-II%20on%20cell%20growth%20and%20oestrogen%20metabolism%20in%20human%20breast%20cancer%20cell%20lines.pdf |
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| author | Wong, Shew Fung Reimann, Karin Lai, Leslie C. |
| author_facet | Wong, Shew Fung Reimann, Karin Lai, Leslie C. |
| author_sort | Wong, Shew Fung |
| collection | UPM |
| description | Oestrogens play an important role in the development of breast cancer. Oestrone sulphate (E1S) acts as a huge reservoir of oestrogens in the breast and is converted to oestrone (E1) by oestrone sulphatase (E1STS). E1 is then reversibly converted to the potent oestrogen, oestradiol (E2) by oestradiol-17β hydroxysteroid dehydrogenase (E2DH). The aim of this study was to assess the effects of transforming growth factor-β1 (TGFβ1), insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II) on cell growth, E1STS and E2DH activities in the MCF-7 and MDA-MB-231 human breast cancer cell lines. TGFβ1, IGF-I and IGF-II alone or in combination inhibited cell growth of both cell lines but no additive or synergistic effects were observed. The treatments significantly stimulated E1STS activity in the MCF-7 cell line, except for TGF1 alone and TGF1 and IGF-I in combination, where no effects were seen. Only TGF1 and IGF-II acted synergistically to stimulate E1STS activity in the MCF-7 cells. There was no significant effect on E1STS activity in the MDA-MB-231 cells with any of the treatments. In the MCF-7 cells, TGFβ1 and IGF-I, IGF-I and IGF-II, and TGFβ1, IGF-I and IGF-II acted synergistically to stimulate the reductive E2DH activity, while only TGFβ1, IGF-I and IGF-II synergistically stimulated the oxidative E2DH activity. There were no additive or synergistic effects on both oxidative and reductive E2DH activities in the MDA-MB-231 cells. In conclusion, TGFβ1, IGF-I and IGF-II may have effects on oestrogen metabolism, especially in the MCF-7 cell line where they stimulated the conversion of E1S to E1 and E1 to E2 and, thus, may have roles to play in the development of breast cancer. |
| first_indexed | 2024-03-06T09:08:40Z |
| format | Article |
| id | upm.eprints-50002 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:08:40Z |
| publishDate | 2001 |
| publisher | Royal College of Pathologists of Australasia |
| record_format | dspace |
| spelling | upm.eprints-500022016-12-30T05:56:45Z http://psasir.upm.edu.my/id/eprint/50002/ Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines Wong, Shew Fung Reimann, Karin Lai, Leslie C. Oestrogens play an important role in the development of breast cancer. Oestrone sulphate (E1S) acts as a huge reservoir of oestrogens in the breast and is converted to oestrone (E1) by oestrone sulphatase (E1STS). E1 is then reversibly converted to the potent oestrogen, oestradiol (E2) by oestradiol-17β hydroxysteroid dehydrogenase (E2DH). The aim of this study was to assess the effects of transforming growth factor-β1 (TGFβ1), insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II) on cell growth, E1STS and E2DH activities in the MCF-7 and MDA-MB-231 human breast cancer cell lines. TGFβ1, IGF-I and IGF-II alone or in combination inhibited cell growth of both cell lines but no additive or synergistic effects were observed. The treatments significantly stimulated E1STS activity in the MCF-7 cell line, except for TGF1 alone and TGF1 and IGF-I in combination, where no effects were seen. Only TGF1 and IGF-II acted synergistically to stimulate E1STS activity in the MCF-7 cells. There was no significant effect on E1STS activity in the MDA-MB-231 cells with any of the treatments. In the MCF-7 cells, TGFβ1 and IGF-I, IGF-I and IGF-II, and TGFβ1, IGF-I and IGF-II acted synergistically to stimulate the reductive E2DH activity, while only TGFβ1, IGF-I and IGF-II synergistically stimulated the oxidative E2DH activity. There were no additive or synergistic effects on both oxidative and reductive E2DH activities in the MDA-MB-231 cells. In conclusion, TGFβ1, IGF-I and IGF-II may have effects on oestrogen metabolism, especially in the MCF-7 cell line where they stimulated the conversion of E1S to E1 and E1 to E2 and, thus, may have roles to play in the development of breast cancer. Royal College of Pathologists of Australasia 2001 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50002/1/Effect%20of%20transforming%20growth%20factor-%CE%B21%2C%20insulin-like%20growth%20factor-I%20and%20insulin-like%20growth%20factor-II%20on%20cell%20growth%20and%20oestrogen%20metabolism%20in%20human%20breast%20cancer%20cell%20lines.pdf Wong, Shew Fung and Reimann, Karin and Lai, Leslie C. (2001) Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines. Pathology, 33 (4). pp. 454-459. ISSN 0031-3025; ESSN: 1465-3931 http://www.sciencedirect.com/science/article/pii/S0031302516345421 10.1080/00313020120083179 |
| spellingShingle | Wong, Shew Fung Reimann, Karin Lai, Leslie C. Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines |
| title | Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines |
| title_full | Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines |
| title_fullStr | Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines |
| title_full_unstemmed | Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines |
| title_short | Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines |
| title_sort | effect of transforming growth factor β1 insulin like growth factor i and insulin like growth factor ii on cell growth and oestrogen metabolism in human breast cancer cell lines |
| url | http://psasir.upm.edu.my/id/eprint/50002/1/Effect%20of%20transforming%20growth%20factor-%CE%B21%2C%20insulin-like%20growth%20factor-I%20and%20insulin-like%20growth%20factor-II%20on%20cell%20growth%20and%20oestrogen%20metabolism%20in%20human%20breast%20cancer%20cell%20lines.pdf |
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