Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit
This study was conducted to determine erythrocyte glutathione peroxidase (GSH-Px) activity, in pregnant Boer does supplemented daily with and without dried Morinda citrifolia fruit, analysed using two different methods; a manual colourimetric method (direct method) and a commercial GSH-Px ELISA kit...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/50234/1/2-14.pdf |
| _version_ | 1825930292714536960 |
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| author | Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil |
| author_facet | Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil |
| author_sort | Alsharif, A. H. K. H. |
| collection | UPM |
| description | This study was conducted to determine erythrocyte glutathione peroxidase (GSH-Px) activity, in pregnant
Boer does supplemented daily with and without dried Morinda citrifolia fruit, analysed using two different methods; a manual colourimetric method (direct method) and a commercial GSH-Px ELISA kit. The does were supplemented with M. citrifolia fruit started from third trimester of pregnancy until 2.5 months post partum. Results showed that GSH-Px analysed using the direct method produced insignificant differences between the groups throughout the experimental period, while the commercial ELISA kit produced some significant differences between the groups during the experiment. These indicate that erythrocyte GSH-Px activity is more stable analysed using the commercial kit and hence provides more accurate results compared to the direct method. |
| first_indexed | 2024-03-06T09:09:23Z |
| format | Conference or Workshop Item |
| id | upm.eprints-50234 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:09:23Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | upm.eprints-502342017-02-02T05:02:27Z http://psasir.upm.edu.my/id/eprint/50234/ Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil This study was conducted to determine erythrocyte glutathione peroxidase (GSH-Px) activity, in pregnant Boer does supplemented daily with and without dried Morinda citrifolia fruit, analysed using two different methods; a manual colourimetric method (direct method) and a commercial GSH-Px ELISA kit. The does were supplemented with M. citrifolia fruit started from third trimester of pregnancy until 2.5 months post partum. Results showed that GSH-Px analysed using the direct method produced insignificant differences between the groups throughout the experimental period, while the commercial ELISA kit produced some significant differences between the groups during the experiment. These indicate that erythrocyte GSH-Px activity is more stable analysed using the commercial kit and hence provides more accurate results compared to the direct method. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50234/1/2-14.pdf Alsharif, A. H. K. H. and Hamzah, Hazilawati and Shaari, Mohd Rosly and Mohamed Mustapha, Noordin and Sithambaram, Shanmugavelu and Haron, Abd Wahid and K., Musaddim and A. M., Nabil (2012) Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 379-381). |
| spellingShingle | Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit |
| title | Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit |
| title_full | Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit |
| title_fullStr | Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit |
| title_full_unstemmed | Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit |
| title_short | Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit |
| title_sort | erythrocyte glutathione peroxidase activity analysis manual colourimetric method versus commercial elisa kit |
| url | http://psasir.upm.edu.my/id/eprint/50234/1/2-14.pdf |
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