Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3
A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular we...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
PeerJ
2016
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Subjects: | |
Online Access: | http://psasir.upm.edu.my/id/eprint/54245/1/Expression%20and%20characterization%20of.pdf |