Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3
A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular we...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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PeerJ
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54245/1/Expression%20and%20characterization%20of.pdf |
| _version_ | 1825931000621826048 |
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| author | Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Thean, Adam Chor Leow Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri |
| author_facet | Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Thean, Adam Chor Leow Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri |
| author_sort | Latip, Wahhida |
| collection | UPM |
| description | A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10-70 °C and stable over a broad pH range from 5.0 to pH 10.0. Various mono and divalent metal ions increased the activity of the AMS3 lipase, but Ni2+ decreased its activity. The purified lipase exhibited the highest activity in the presence of sunflower oil. In addition, the enzyme activity in 25% v/v solvents at 50 °C particularly to n-hexane, DMSO and methanol could be useful for catalysis reaction in organic solvent and at broad temperature. |
| first_indexed | 2024-03-06T09:20:06Z |
| format | Article |
| id | upm.eprints-54245 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:20:06Z |
| publishDate | 2016 |
| publisher | PeerJ |
| record_format | dspace |
| spelling | upm.eprints-542452018-03-08T04:40:21Z http://psasir.upm.edu.my/id/eprint/54245/ Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Thean, Adam Chor Leow Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10-70 °C and stable over a broad pH range from 5.0 to pH 10.0. Various mono and divalent metal ions increased the activity of the AMS3 lipase, but Ni2+ decreased its activity. The purified lipase exhibited the highest activity in the presence of sunflower oil. In addition, the enzyme activity in 25% v/v solvents at 50 °C particularly to n-hexane, DMSO and methanol could be useful for catalysis reaction in organic solvent and at broad temperature. PeerJ 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54245/1/Expression%20and%20characterization%20of.pdf Latip, Wahhida and Raja Abd Rahman, Raja Noor Zaliha and Thean, Adam Chor Leow and Mohd Shariff, Fairolniza and Mohamad Ali, Mohd Shukuri (2016) Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3. PeerJ, 4. pp. 1-20. ISSN 2167-8359 https://peerj.com/articles/2420/ Thermotolerant lipase; Expression and characterization 10.7717/peerj.2420 |
| spellingShingle | Thermotolerant lipase; Expression and characterization Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Thean, Adam Chor Leow Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 |
| title | Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 |
| title_full | Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 |
| title_fullStr | Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 |
| title_full_unstemmed | Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 |
| title_short | Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 |
| title_sort | expression and characterization of thermotolerant lipase with broad ph profiles isolated from an antarctic pseudomonas sp strain ams3 |
| topic | Thermotolerant lipase; Expression and characterization |
| url | http://psasir.upm.edu.my/id/eprint/54245/1/Expression%20and%20characterization%20of.pdf |
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