Recovery of histidine-tagged nucleocapsid protein of Newcastle disease virus using immobilised metal affinity chromatography
An immobilised metal affinity packed bed adsorption chromatography (IMA-PBAC) for the purification of recombinant nucleocapsid protein (NP) of Newcastle disease virus (NDV) directly from clarified feedstock was developed. The XK 16/20 (i.d. = 16 mm) was used as a packed bed column and Streamline che...
Main Authors: | , , , , |
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Format: | Article |
Language: | English English |
Published: |
Elsevier
2006
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Online Access: | http://psasir.upm.edu.my/id/eprint/5608/1/Recovery%20of%20histidine.pdf |