Recovery of histidine-tagged nucleocapsid protein of Newcastle disease virus using immobilised metal affinity chromatography

An immobilised metal affinity packed bed adsorption chromatography (IMA-PBAC) for the purification of recombinant nucleocapsid protein (NP) of Newcastle disease virus (NDV) directly from clarified feedstock was developed. The XK 16/20 (i.d. = 16 mm) was used as a packed bed column and Streamline che...

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Bibliographic Details
Main Authors: Tan, Yan Peng, Tau, Chuan Ling, Tan, Wen Siang, Yusoff, Khatijah, Tey, Beng Ti
Format: Article
Language:English
English
Published: Elsevier 2006
Online Access:http://psasir.upm.edu.my/id/eprint/5608/1/Recovery%20of%20histidine.pdf