Reductive alkylation of lipase: experimental and molecular modeling approaches

Candida rugosa lipase was modified via reductive alkylation to increase its hydrophobicity to work better in organic solvents. The free amino group of lysines was alkylated using propionaldehyde with different degrees of modification obtained (49 and 86%). Far-ultraviolet circular dichroism (CD) spe...

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Bibliographic Details
Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Tejo, Bimo Ario, Basri, Mahiran, Abdul Rahman, Mohd Basyaruddin, Khan, Farid, Md Zain, Sharifuddin, Siahaan, Teruna J., Salleh, Abu Bakar
Format: Article
Language:English
English
Published: Humana Press 2004
Online Access:http://psasir.upm.edu.my/id/eprint/56252/1/56252.pdf
http://psasir.upm.edu.my/id/eprint/56252/7/1-s2.0-014102299190097T-main.pdf