Reductive alkylation of lipase: experimental and molecular modeling approaches
Candida rugosa lipase was modified via reductive alkylation to increase its hydrophobicity to work better in organic solvents. The free amino group of lysines was alkylated using propionaldehyde with different degrees of modification obtained (49 and 86%). Far-ultraviolet circular dichroism (CD) spe...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Humana Press
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56252/1/56252.pdf http://psasir.upm.edu.my/id/eprint/56252/7/1-s2.0-014102299190097T-main.pdf |