Expression, Purification And Characterization Of Organic Solvent Tolerant Lipase From Bacillus Sphaericus 205y
One thousand and two hundred base pairs (bp) of open reading frame (ORF) encoding for an organic solvent tolerant lipase gene was cloned and expressed intra- and extracellularly. The intracellular expression was done using pBAD TOPO TA expression vector with 0.02% (vlv) of L-arabinose as optimum...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2005
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/5905/1/FBSB_2005_6%20IR.pdf |