Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, sca...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62159/1/Influence%20of%20high-pressure%20steam%20pretreatment%20on%20the%20structure%20of%20rice%20husk%20and%20enzymatic%20saccharification%20in%20a%20two-step%20system.pdf |
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| author | Kazeem, Muinat Olanike Md Shah, Umi Kalsom Samsu Baharuddin, Azhari Abdul Rahman, Nor' Aini |
| author_facet | Kazeem, Muinat Olanike Md Shah, Umi Kalsom Samsu Baharuddin, Azhari Abdul Rahman, Nor' Aini |
| author_sort | Kazeem, Muinat Olanike |
| collection | UPM |
| description | This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analyses. Optimum pretreatment conditions (200 °C, 1.85 MPa for 7 min), enzyme concentration at 30 FPU/g and temperature at 60 °C for 48 h of continuous saccharification effectively produced sugar (21.1 g/L = 0.422 g/g dry substrate) at a saccharification degree of 53.87%. Conducting a second-step enzymatic saccharification resulted in additional sugar production (7.9 g/L = 0.158 g/g substrate) and a 20.44% saccharification degree. In contrast, the two-step saccharification process (48 and 24 h) achieved optimal sugar yield of 0.581 g/g substrate and saccharification degree of 73.5%. Additionally, the process improved the yield of monomeric sugars of glucose (0.465 g/g), xylose (0.010 g/g), and cellobiose (0.063 g/g). Therefore, the combination of the high-pressure steam pretreatment with thermostable cellulase from Bacillus licheniformis 2D55 in a two-step enzymatic saccharification process is an economically viable method in rice husk bioprocessing for sugar production. |
| first_indexed | 2024-03-06T09:42:13Z |
| format | Article |
| id | upm.eprints-62159 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:42:13Z |
| publishDate | 2017 |
| publisher | North Carolina State University |
| record_format | dspace |
| spelling | upm.eprints-621592019-04-17T08:37:32Z http://psasir.upm.edu.my/id/eprint/62159/ Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system Kazeem, Muinat Olanike Md Shah, Umi Kalsom Samsu Baharuddin, Azhari Abdul Rahman, Nor' Aini This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analyses. Optimum pretreatment conditions (200 °C, 1.85 MPa for 7 min), enzyme concentration at 30 FPU/g and temperature at 60 °C for 48 h of continuous saccharification effectively produced sugar (21.1 g/L = 0.422 g/g dry substrate) at a saccharification degree of 53.87%. Conducting a second-step enzymatic saccharification resulted in additional sugar production (7.9 g/L = 0.158 g/g substrate) and a 20.44% saccharification degree. In contrast, the two-step saccharification process (48 and 24 h) achieved optimal sugar yield of 0.581 g/g substrate and saccharification degree of 73.5%. Additionally, the process improved the yield of monomeric sugars of glucose (0.465 g/g), xylose (0.010 g/g), and cellobiose (0.063 g/g). Therefore, the combination of the high-pressure steam pretreatment with thermostable cellulase from Bacillus licheniformis 2D55 in a two-step enzymatic saccharification process is an economically viable method in rice husk bioprocessing for sugar production. North Carolina State University 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62159/1/Influence%20of%20high-pressure%20steam%20pretreatment%20on%20the%20structure%20of%20rice%20husk%20and%20enzymatic%20saccharification%20in%20a%20two-step%20system.pdf Kazeem, Muinat Olanike and Md Shah, Umi Kalsom and Samsu Baharuddin, Azhari and Abdul Rahman, Nor' Aini (2017) Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system. BioResources, 12 (3). pp. 6207-6236. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_3_6207_Kazeem_High_Pressure_Steam_Pretreatment_Rice_Husk |
| spellingShingle | Kazeem, Muinat Olanike Md Shah, Umi Kalsom Samsu Baharuddin, Azhari Abdul Rahman, Nor' Aini Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system |
| title | Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system |
| title_full | Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system |
| title_fullStr | Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system |
| title_full_unstemmed | Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system |
| title_short | Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system |
| title_sort | influence of high pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two step system |
| url | http://psasir.upm.edu.my/id/eprint/62159/1/Influence%20of%20high-pressure%20steam%20pretreatment%20on%20the%20structure%20of%20rice%20husk%20and%20enzymatic%20saccharification%20in%20a%20two-step%20system.pdf |
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