Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach

BACKGROUND: Quantitative PCR (qPCR) is a common method for quantifying mRNA expression. Given the heterogeneity present in tumor tissues, it is crucial to normalize target mRNA expression data using appropriate reference genes that are stably expressed under a variety of pathological and experimenta...

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Bibliographic Details
Main Authors: Selvarajah, Gayathri Thevi, Bonestroo, Floor A. S., Timmermans Sprang, Elpetra P. M., Kirpensteijn, Jolle, Mol, Jan A.
Format: Article
Language:English
Published: BioMed Central 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62931/1/Reference%20gene%20validation%20for%20gene%20expression%20normalization%20in%20canine%20osteosarcoma.pdf