Rapid detection of Newcastle disease virus (NDV) with SYBR Green I real-time polymerase chain reaction
A two-step real-time PCR assay for the detection of Newcastle disease virus (NDV) was developed. Twelve NDV isolates (7 velogenic, 1 mesogenic and 4 lentogenic strains) were used. All the isolates showed positive results in amplification. Specificity of the amplification was confirmed by melting cur...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Universiti Putra Malaysia Press
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/65111/1/71-20.pdf |