Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction
A two-step SYBR Green I real time polymerase chain reaction (PCR, real time PCR) for the detection of Newcastle disease virus (NDV) was developed. A melting curve analysis was performed to distinguish specific from non-specific products and primer dimmers. Regardless of different virus pathotypes t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AEPress
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/692/1/PFBSB8.pdf |