Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction

A two-step SYBR Green I real time polymerase chain reaction (PCR, real time PCR) for the detection of Newcastle disease virus (NDV) was developed. A melting curve analysis was performed to distinguish specific from non-specific products and primer dimmers. Regardless of different virus pathotypes t...

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Détails bibliographiques
Auteurs principaux: Tan, Sheau Wei, Omar, Abdul Rahman, Ideris, Aini, Yusoff, Khatijah, Tan, Wen Siang
Format: Article
Langue:English
Publié: AEPress 2004
Accès en ligne:http://psasir.upm.edu.my/id/eprint/692/1/PFBSB8.pdf