Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch
Pullulanase (EC.3.2.1.41) was used to generate more linear-chain dextrin from sago starch (24.9% amylose) such that the resulting product could act as a high amylose starch. A starch suspension of 5.0% (w/v) sago starch was heated at 100 °C for 45 min and, after cooling, the gelatinized sago starch...
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| Format: | Article |
| Language: | English English |
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Copyright © 2005 Elsevier Ltd
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7173/1/Enzymatic%20production%20of%20linear%20long.pdf |
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| author | Wong, Chen Wai Syed Muhammad, Sharifah Kharidah Mat Hashim, Dzulkifly Saari, Nazamid Mohd Ghazali, Hasanah |
| author_facet | Wong, Chen Wai Syed Muhammad, Sharifah Kharidah Mat Hashim, Dzulkifly Saari, Nazamid Mohd Ghazali, Hasanah |
| author_sort | Wong, Chen Wai |
| collection | UPM |
| description | Pullulanase (EC.3.2.1.41) was used to generate more linear-chain dextrin from sago starch (24.9% amylose) such that the resulting product could act as a high amylose starch. A starch suspension of 5.0% (w/v) sago starch was heated at 100 °C for 45 min and, after cooling, the gelatinized sago starch was hydrolyzed with 2.0% (v/dry weight starch) pullulanase (Promozyme 400L, Novozymes A/S, Denmark) for 24 h. The linear long-chain dextrin (LLD) content of the hydrolysate after drying, was then compared with the initial LLD content. The surface morphology of the starch granules was observed with a scanning electron microscope (SEM). The effects of gelatinization, time of reaction, pretreatment with different strengths of hydrochloric acid prior to enzyme hydrolysis, and starch and enzyme concentrations were studied. Raw sago starch was resistant to the action of pullulanase, but caused an increase in the LLD of that sago starch from an initial concentration of 24.9–33.2% following gelatinization. The best conditions to maximize the amount of LLD were 5.0% (w/v) sago starch, 2.0% (v/w) enzyme and 12 h reaction time. Acid pretreatment of the sago starch did not cause greater improvement in the accessibility and susceptibility of pullulanase as the LLD content, following pullulanase action did not change significantly. Shrinkage on the surface of the starch granules was observed with the SEM. |
| first_indexed | 2024-03-06T07:11:27Z |
| format | Article |
| id | upm.eprints-7173 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:11:27Z |
| publishDate | 2007 |
| publisher | Copyright © 2005 Elsevier Ltd |
| record_format | dspace |
| spelling | upm.eprints-71732015-10-22T02:23:46Z http://psasir.upm.edu.my/id/eprint/7173/ Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch Wong, Chen Wai Syed Muhammad, Sharifah Kharidah Mat Hashim, Dzulkifly Saari, Nazamid Mohd Ghazali, Hasanah Pullulanase (EC.3.2.1.41) was used to generate more linear-chain dextrin from sago starch (24.9% amylose) such that the resulting product could act as a high amylose starch. A starch suspension of 5.0% (w/v) sago starch was heated at 100 °C for 45 min and, after cooling, the gelatinized sago starch was hydrolyzed with 2.0% (v/dry weight starch) pullulanase (Promozyme 400L, Novozymes A/S, Denmark) for 24 h. The linear long-chain dextrin (LLD) content of the hydrolysate after drying, was then compared with the initial LLD content. The surface morphology of the starch granules was observed with a scanning electron microscope (SEM). The effects of gelatinization, time of reaction, pretreatment with different strengths of hydrochloric acid prior to enzyme hydrolysis, and starch and enzyme concentrations were studied. Raw sago starch was resistant to the action of pullulanase, but caused an increase in the LLD of that sago starch from an initial concentration of 24.9–33.2% following gelatinization. The best conditions to maximize the amount of LLD were 5.0% (w/v) sago starch, 2.0% (v/w) enzyme and 12 h reaction time. Acid pretreatment of the sago starch did not cause greater improvement in the accessibility and susceptibility of pullulanase as the LLD content, following pullulanase action did not change significantly. Shrinkage on the surface of the starch granules was observed with the SEM. Copyright © 2005 Elsevier Ltd 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7173/1/Enzymatic%20production%20of%20linear%20long.pdf Wong, Chen Wai and Syed Muhammad, Sharifah Kharidah and Mat Hashim, Dzulkifly and Saari, Nazamid and Mohd Ghazali, Hasanah (2007) Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch. Food Chemistry, 100 (2). pp. 774-780. ISSN 0308-8146 http://dx.doi.org/10.1016/j.foodchem.2005.10.040 Pullulanase Sago - Hydrolysis Dextrins 10.1016/j.foodchem.2005.10.040 English |
| spellingShingle | Pullulanase Sago - Hydrolysis Dextrins Wong, Chen Wai Syed Muhammad, Sharifah Kharidah Mat Hashim, Dzulkifly Saari, Nazamid Mohd Ghazali, Hasanah Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch |
| title | Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch |
| title_full | Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch |
| title_fullStr | Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch |
| title_full_unstemmed | Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch |
| title_short | Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch |
| title_sort | enzymatic production of linear long chain dextrin from sago metroxylon sagu starch |
| topic | Pullulanase Sago - Hydrolysis Dextrins |
| url | http://psasir.upm.edu.my/id/eprint/7173/1/Enzymatic%20production%20of%20linear%20long.pdf |
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