An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization

The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K pr...

Full description

Bibliographic Details
Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Lee, Poh Geok, Basri, Mahiran, Salleh, Abu Bakar
Format: Article
Language:English
Published: Elsevier 2006
Online Access:http://psasir.upm.edu.my/id/eprint/7245/1/1An%20organic%20solvent.pdf