An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization
The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K pr...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2006
|
Online Access: | http://psasir.upm.edu.my/id/eprint/7245/1/1An%20organic%20solvent.pdf |