An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization
The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K pr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7245/1/1An%20organic%20solvent.pdf |
| _version_ | 1796967283647578112 |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| collection | UPM |
| description | The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K protease was an alkaline metalloprotease with an optimum pH and temperature of 10 and 70 °C, respectively. The enzyme showed stability and activation in the presence of organic solvents with log Pa/w values equal or more than 4.0. After 14 days of incubation, the purified protease was activated 1.11, 1.82, 1.50, 1.75 and 1.80 times in 1-decanol, isooctane, decane, dodecane and hexadecane, respectively. |
| first_indexed | 2024-03-06T07:11:40Z |
| format | Article |
| id | upm.eprints-7245 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:11:40Z |
| publishDate | 2006 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-72452016-09-26T08:53:36Z http://psasir.upm.edu.my/id/eprint/7245/ An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar The organic solvent-tolerant strain K protease was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatography with 124-fold increase in specific activity. The molecular mass of the purified enzyme as revealed by SDS-PAGE electrophoresis is 51,000 Da. The strain K protease was an alkaline metalloprotease with an optimum pH and temperature of 10 and 70 °C, respectively. The enzyme showed stability and activation in the presence of organic solvents with log Pa/w values equal or more than 4.0. After 14 days of incubation, the purified protease was activated 1.11, 1.82, 1.50, 1.75 and 1.80 times in 1-decanol, isooctane, decane, dodecane and hexadecane, respectively. Elsevier 2006-11-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7245/1/1An%20organic%20solvent.pdf Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2006) An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization. Enzyme and Microbial Technology, 39 (7). pp. 1484-1491. ISSN 0141-0229; ESSN: 1879-0909 http://www.sciencedirect.com/science/article/pii/S0141022906001803 10.1016/j.enzmictec.2006.03.038 |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization |
| title | An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization |
| title_full | An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization |
| title_fullStr | An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization |
| title_full_unstemmed | An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization |
| title_short | An organic solvent-stable alkaline protease from Pseudomonas aeruginosa strain K: enzyme purification and characterization |
| title_sort | organic solvent stable alkaline protease from pseudomonas aeruginosa strain k enzyme purification and characterization |
| url | http://psasir.upm.edu.my/id/eprint/7245/1/1An%20organic%20solvent.pdf |
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