Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems

Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems

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The enhanced green fluorescent protein (EGFP) is widely used as a marker in life science. Currently, purifications of the EGFP mostly involve chromatographic methods, which are multistep, time-consuming and costly. In the present study, the recombinant EGFP expressed in Escherichia coli was purified...

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Bibliographic Details
Main Authors: Sewn, Cen Lo, Ramanan, Ramakrishnan Nagasundara, Tey, Beng Ti, Tan, Wen Siang, Pau, Loke Show, Tau, Chuan Ling, Chien, Wei Ooi
Format: Article
Language:English
Published: Elsevier 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73684/1/Purification%20of%20the%20recombinant%20enhanced%20green%20fluorescent%20protein%20from%20Escherichia%20coli%20using%20alcohol%20%2B%20salt%20aqueous%20two-phase%20systems.pdf

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http://psasir.upm.edu.my/id/eprint/73684/1/Purification%20of%20the%20recombinant%20enhanced%20green%20fluorescent%20protein%20from%20Escherichia%20coli%20using%20alcohol%20%2B%20salt%20aqueous%20two-phase%20systems.pdf

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