Purification, crystallization and molecular modeling of recombinant A3 lipase from an antartic Pseudomonas sp. strain AMS3

A recombinant A3 lipase from Pseudomonas sp. strain AMS 3 was previously expressed in E. coli. In present study, recombinant A3 lipase was successfully purified through Nickel-Sepharose Fast Flow affinity chromatography. This purification recovered 58.47% of yield with the purification fold 3.69. So...

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Bibliographic Details
Main Author: Leong, Mun Hong
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/78207/1/FBSB%202015%2056%20-%20IR.pdf