Effects of lid 1 mutagenesis on lid displacement, catalytic performances and thermostability of cold-active Pseudomonas AMS8 lipase in toluene
Pseudomonas fluorescens AMS8 lipase lid 1 structure is rigid and holds unclear roles due to the absence of solvent-interactions. Lid 1 region was stabilized by 17 hydrogen bond linkages and displayed lower mean hydrophobicity (0.596) compared to MIS38 lipase. Mutating lid 1 residues, Thr-52 and Gly-...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79602/1/Effects%20of%20lid%201%20mutagenesis%20on%20lid%20displacement.pdf |