Optimization of qPCR assay of chicken cytokine genes using sybr green method

Quantitative PCR (qPCR) has been widely utilized in the study of cytokine expression profile upon vaccination of recombinant fowlpox virus and challenge of avian influenza virus. Although Taqman qPCR method offers specific detection of amplified products, SYBR green qPCR is more cost effective and r...

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Bibliographic Details
Main Author: Chan, Jia Hui
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/91031/1/FBSB%202015%20141%20-%20IR.pdf