Site-directed mutagenesis of helical lid of J15 lipase

Lid of lipases modulate the specificity of the substrate to the catalytic active site of lipase by interfacial activation. To address the role of lid on the substrate specificity, site-directed mutagenesis was used to modify the hydrophobicity of the lid by computer simulation and experimental ap...

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Bibliographic Details
Main Author: Ho, Chern Min
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/91037/1/FBSB%202015%20147%20-%20IR%27.pdf