Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications
Keratinase is an important enzyme that can degrade recalcitrant keratinous wastes to form beneficial recyclable keratin hydrolysates. Keratinase is not only important as an alternative to reduce environmental pollution caused by chemical treatments of keratinous wastes, but it also has industrial si...
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| Format: | Article |
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Springer
2021
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| _version_ | 1825937429827158016 |
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| author | Radin Yahaya, Radin Shafierul Normi, Yahaya M. Lai, Yee Phang Ahmad, Siti Aqlima Ong Abdullah, Janna Sabri, Suriana |
| author_facet | Radin Yahaya, Radin Shafierul Normi, Yahaya M. Lai, Yee Phang Ahmad, Siti Aqlima Ong Abdullah, Janna Sabri, Suriana |
| author_sort | Radin Yahaya, Radin Shafierul |
| collection | UPM |
| description | Keratinase is an important enzyme that can degrade recalcitrant keratinous wastes to form beneficial recyclable keratin hydrolysates. Keratinase is not only important as an alternative to reduce environmental pollution caused by chemical treatments of keratinous wastes, but it also has industrial significance. Currently, the bioproduction of keratinase from native keratinolytic host is considered low, and this hampers large-scale usage of the enzyme. Straightforward approaches of cloning and expression of recombinant keratinases from native keratinolytic host are employed to elevate the amount of keratinase produced. However, this is still insufficient to compensate for the lack of its large-scale production to meet the industrial demands. Hence, this review aimed to highlight the various sources of keratinase and the strategies to increase its production in native keratinolytic hosts. Molecular strategies to increase the production of recombinant keratinase such as plasmid selection, promoter engineering, chromosomal integration, signal peptide and propeptide engineering, codon optimization, and glycoengineering were also described. These mentioned strategies have been utilized in heterologous expression hosts, namely, Escherichia coli, Bacillus sp., and Pichia pastoris, as they are most widely used for the heterologous propagations of keratinases to further intensify the production of recombinant keratinases adapted to better suit the large-scale demand for them. |
| first_indexed | 2024-03-06T10:59:22Z |
| format | Article |
| id | upm.eprints-94258 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:59:22Z |
| publishDate | 2021 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-942582023-05-08T04:12:20Z http://psasir.upm.edu.my/id/eprint/94258/ Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications Radin Yahaya, Radin Shafierul Normi, Yahaya M. Lai, Yee Phang Ahmad, Siti Aqlima Ong Abdullah, Janna Sabri, Suriana Keratinase is an important enzyme that can degrade recalcitrant keratinous wastes to form beneficial recyclable keratin hydrolysates. Keratinase is not only important as an alternative to reduce environmental pollution caused by chemical treatments of keratinous wastes, but it also has industrial significance. Currently, the bioproduction of keratinase from native keratinolytic host is considered low, and this hampers large-scale usage of the enzyme. Straightforward approaches of cloning and expression of recombinant keratinases from native keratinolytic host are employed to elevate the amount of keratinase produced. However, this is still insufficient to compensate for the lack of its large-scale production to meet the industrial demands. Hence, this review aimed to highlight the various sources of keratinase and the strategies to increase its production in native keratinolytic hosts. Molecular strategies to increase the production of recombinant keratinase such as plasmid selection, promoter engineering, chromosomal integration, signal peptide and propeptide engineering, codon optimization, and glycoengineering were also described. These mentioned strategies have been utilized in heterologous expression hosts, namely, Escherichia coli, Bacillus sp., and Pichia pastoris, as they are most widely used for the heterologous propagations of keratinases to further intensify the production of recombinant keratinases adapted to better suit the large-scale demand for them. Springer 2021-05-03 Article PeerReviewed Radin Yahaya, Radin Shafierul and Normi, Yahaya M. and Lai, Yee Phang and Ahmad, Siti Aqlima and Ong Abdullah, Janna and Sabri, Suriana (2021) Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications. Applied Microbiology and Biotechnology, 105. 3955 - 3969. ISSN 0175-7598; ESSN: 432-0614 https://link.springer.com/article/10.1007/s00253-021-11321-y 10.1007/s00253-021-11321-y |
| spellingShingle | Radin Yahaya, Radin Shafierul Normi, Yahaya M. Lai, Yee Phang Ahmad, Siti Aqlima Ong Abdullah, Janna Sabri, Suriana Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications |
| title | Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications |
| title_full | Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications |
| title_fullStr | Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications |
| title_full_unstemmed | Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications |
| title_short | Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications |
| title_sort | molecular strategies to increase keratinase production in heterologous expression systems for industrial applications |
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