Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae
5M mutant lipase was derived through cumulative mutagenesis of amino acid residues (D43E/T118N/E226D/E250L/N304E) of T1 lipase from Geobacillus zalihae. A previous study revealed that cumulative mutations in 5M mutant lipase resulted in decreased thermostability compared to wild-type T1 lipase. Mult...
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Public Library of Science
2021
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