Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines
The formation of two nanodelivery systems, Sorafenib (SF)-loaded chitosan (SF-CS) and their folate-coated (SF-CS-FA) nanoparticles (NPs), were developed to enhance SF drug delivery on human Hepatocellular Carcinoma (HepG2) and Colorectal Adenocarcinoma (HT29) cell lines. The ionic gelation method wa...
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| Format: | Article |
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MDPI AG
2021
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| author | Ruman, Umme Buskaran, Kalaivani Pastorin, Giorgia Masarudin, Mas Jaffri Fakurazi, Sharida Hussein, Mohd Zobir |
| author_facet | Ruman, Umme Buskaran, Kalaivani Pastorin, Giorgia Masarudin, Mas Jaffri Fakurazi, Sharida Hussein, Mohd Zobir |
| author_sort | Ruman, Umme |
| collection | UPM |
| description | The formation of two nanodelivery systems, Sorafenib (SF)-loaded chitosan (SF-CS) and their folate-coated (SF-CS-FA) nanoparticles (NPs), were developed to enhance SF drug delivery on human Hepatocellular Carcinoma (HepG2) and Colorectal Adenocarcinoma (HT29) cell lines. The ionic gelation method was adopted to synthesize the NPs. The characterizations were performed by DLS, FESEM, TEM, XRD, TGA, FTIR, and UV-visible spectroscopy. It was found that 83.7 ± 2.4% and 87.9 ± 1.1% of encapsulation efficiency; 18.2 ± 1.3% and 19.9 ± 1.4% of loading content; 76.3 ± 13.7 nm and 81.6 ± 12.9 nm of hydrodynamic size; 60–80 nm and 70–100 nm of TEM; and FESEM sizes of near-spherical shape were observed, respectively, for SF-CS and SF-CS-FA nanoparticles. The SF showed excellent release from the nanoparticles under pH 4.8 PBS solution, indicating a good delivery system for tumor cells. The cytotoxicity study revealed their better anticancer action towards HepG2 and HT29 cell lines compared to the free sorafenib. Moreover, both NPs systems showed negligible toxicity to normal Human Dermal Fibroblast adult cells (HDFa). This is towards an enhanced anticancer drug delivery system with sustained-release properties for better cancer management. |
| first_indexed | 2024-03-06T11:01:04Z |
| format | Article |
| id | upm.eprints-95251 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:01:04Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | upm.eprints-952512023-01-31T04:13:23Z http://psasir.upm.edu.my/id/eprint/95251/ Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines Ruman, Umme Buskaran, Kalaivani Pastorin, Giorgia Masarudin, Mas Jaffri Fakurazi, Sharida Hussein, Mohd Zobir The formation of two nanodelivery systems, Sorafenib (SF)-loaded chitosan (SF-CS) and their folate-coated (SF-CS-FA) nanoparticles (NPs), were developed to enhance SF drug delivery on human Hepatocellular Carcinoma (HepG2) and Colorectal Adenocarcinoma (HT29) cell lines. The ionic gelation method was adopted to synthesize the NPs. The characterizations were performed by DLS, FESEM, TEM, XRD, TGA, FTIR, and UV-visible spectroscopy. It was found that 83.7 ± 2.4% and 87.9 ± 1.1% of encapsulation efficiency; 18.2 ± 1.3% and 19.9 ± 1.4% of loading content; 76.3 ± 13.7 nm and 81.6 ± 12.9 nm of hydrodynamic size; 60–80 nm and 70–100 nm of TEM; and FESEM sizes of near-spherical shape were observed, respectively, for SF-CS and SF-CS-FA nanoparticles. The SF showed excellent release from the nanoparticles under pH 4.8 PBS solution, indicating a good delivery system for tumor cells. The cytotoxicity study revealed their better anticancer action towards HepG2 and HT29 cell lines compared to the free sorafenib. Moreover, both NPs systems showed negligible toxicity to normal Human Dermal Fibroblast adult cells (HDFa). This is towards an enhanced anticancer drug delivery system with sustained-release properties for better cancer management. MDPI AG 2021-02-16 Article PeerReviewed Ruman, Umme and Buskaran, Kalaivani and Pastorin, Giorgia and Masarudin, Mas Jaffri and Fakurazi, Sharida and Hussein, Mohd Zobir (2021) Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines. Nanomaterials, 11 (2). pp. 1-26. ISSN 1758-1745; ESSN: 2079-4991 https://www.mdpi.com/2079-4991/11/2/497/htm 10.3390/nano11020497 |
| spellingShingle | Ruman, Umme Buskaran, Kalaivani Pastorin, Giorgia Masarudin, Mas Jaffri Fakurazi, Sharida Hussein, Mohd Zobir Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines |
| title | Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines |
| title_full | Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines |
| title_fullStr | Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines |
| title_full_unstemmed | Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines |
| title_short | Synthesis and characterization of chitosan-based nanodelivery systems to enhance the anticancer effect of Sorafenib drug in Hepatocellular Carcinoma and Colorectal Adenocarcinoma cell lines |
| title_sort | synthesis and characterization of chitosan based nanodelivery systems to enhance the anticancer effect of sorafenib drug in hepatocellular carcinoma and colorectal adenocarcinoma cell lines |
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