Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study
A study was performed to detect the viral conjunctivitis in HUSM caused by HSV using a PCR method. Seventy patients with viral conjunctivitis were examined; with clinical features identified and conjunctival scrapping taken from superior and inferior fornices of affected eye for PCR analysis. PCR w...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2005
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| Online Access: | http://eprints.usm.my/42628/1/GP...Detection_Of_Herpes_Simplex_Onfection_In_Viral_Conjuctivis_Using_Polymerase_Chain_Reaction_-_A_Pilot_Study...2005...OCR....pdf |
| _version_ | 1825834654726356992 |
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| author | Alwi, Azma Azalina Ahmad Hussein, Elias Gopalakrishnan, V. Ravichandran, M. |
| author_facet | Alwi, Azma Azalina Ahmad Hussein, Elias Gopalakrishnan, V. Ravichandran, M. |
| author_sort | Alwi, Azma Azalina Ahmad |
| collection | USM |
| description | A study was performed to detect the viral conjunctivitis in HUSM caused by HSV using a PCR method. Seventy patients with viral conjunctivitis were examined; with clinical
features identified and conjunctival scrapping taken from superior and inferior fornices of affected eye for PCR analysis. PCR was performed with primers obtained from a
commercially available primer kit for HSV. The prevalence of viral conjunctivitis in HUSM caused by HSV infection, using PCR method, was 17.I % (95 % CI = 8.1, 26.0) which was higher than other reported studies. This is mainly due to the method used which is a highly sensitive and specific diagnostic test. Majority of HSV conjunctivitis patients presented with moderate follicular conjunctivitis with frequent corneal involvement which was similar to features of adenoviral conjunctivitis caused by Group D subgenera. The possible differentiating feature of adenoviral conjunctivitis was the unilaterality. |
| first_indexed | 2024-03-06T15:25:57Z |
| format | Conference or Workshop Item |
| id | usm.eprints-42628 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:25:57Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | usm.eprints-426282020-03-22T08:03:49Z http://eprints.usm.my/42628/ Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study Alwi, Azma Azalina Ahmad Hussein, Elias Gopalakrishnan, V. Ravichandran, M. RA643-645 Disease (Communicable and noninfectious) and public health A study was performed to detect the viral conjunctivitis in HUSM caused by HSV using a PCR method. Seventy patients with viral conjunctivitis were examined; with clinical features identified and conjunctival scrapping taken from superior and inferior fornices of affected eye for PCR analysis. PCR was performed with primers obtained from a commercially available primer kit for HSV. The prevalence of viral conjunctivitis in HUSM caused by HSV infection, using PCR method, was 17.I % (95 % CI = 8.1, 26.0) which was higher than other reported studies. This is mainly due to the method used which is a highly sensitive and specific diagnostic test. Majority of HSV conjunctivitis patients presented with moderate follicular conjunctivitis with frequent corneal involvement which was similar to features of adenoviral conjunctivitis caused by Group D subgenera. The possible differentiating feature of adenoviral conjunctivitis was the unilaterality. 2005 Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.usm.my/42628/1/GP...Detection_Of_Herpes_Simplex_Onfection_In_Viral_Conjuctivis_Using_Polymerase_Chain_Reaction_-_A_Pilot_Study...2005...OCR....pdf Alwi, Azma Azalina Ahmad and Hussein, Elias and Gopalakrishnan, V. and Ravichandran, M. (2005) Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study. In: Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study. (Submitted) |
| spellingShingle | RA643-645 Disease (Communicable and noninfectious) and public health Alwi, Azma Azalina Ahmad Hussein, Elias Gopalakrishnan, V. Ravichandran, M. Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study |
| title | Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study |
| title_full | Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study |
| title_fullStr | Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study |
| title_full_unstemmed | Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study |
| title_short | Detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction- a pilot study |
| title_sort | detection of herpes simplex infection in viral conjunctivitis using polymerase chain reaction a pilot study |
| topic | RA643-645 Disease (Communicable and noninfectious) and public health |
| url | http://eprints.usm.my/42628/1/GP...Detection_Of_Herpes_Simplex_Onfection_In_Viral_Conjuctivis_Using_Polymerase_Chain_Reaction_-_A_Pilot_Study...2005...OCR....pdf |
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