Manipulation of the conformation and enzymatic properties of T1 Lipase by site-directed mutagenesis of the protein core
In silico and experimental investigations were conducted to explore the effects of substituting hydrophobic residues, Val, Met, Leu, Ile, Trp, and Phe into Gln 114 of T1 lipase. The in silico investigations accurately predicted the enzymatic characteristics of the mutants in the experimental studies...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer
2012
|
| Subjects: |