Domain replacement to elucidate the role of B domain in cgtase thermostability and activity
The B domain of CGTase has been generally accepted as a domain involved in thermostability. However, limited work has been performed in which entire B domain is substituted with the thermostable counterpart. Using overlap extension PCR, we replaced the B domain of a variant of CGTase Bacillus sp. G1...
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| Format: | Article |
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Elsevier
2012
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