Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis
Electrospinning was used to fabricate beadless microfibrous polyacrylonitrile (ePAN) mats with an average fiber diameter of 1448 ± 380 nm from a 10 wt.% PAN in dimethylformamide (DMF) dope solution at applied voltage of 18 kV and 20 cm fiber collection distance. Urease (EC 3.5.1.5) was then covalent...
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| Format: | Article |
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Elsevier
2015
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| author | Daneshfar, Aref Matsuura, Takeshi Emadzadeh, Daryoush Pahlevani, Zahra Ismail, Ahmad Fauzi |
| author_facet | Daneshfar, Aref Matsuura, Takeshi Emadzadeh, Daryoush Pahlevani, Zahra Ismail, Ahmad Fauzi |
| author_sort | Daneshfar, Aref |
| collection | ePrints |
| description | Electrospinning was used to fabricate beadless microfibrous polyacrylonitrile (ePAN) mats with an average fiber diameter of 1448 ± 380 nm from a 10 wt.% PAN in dimethylformamide (DMF) dope solution at applied voltage of 18 kV and 20 cm fiber collection distance. Urease (EC 3.5.1.5) was then covalently immobilized on dispersed microfibrous ePAN mats following the chemical treatment of fibers with ethylenediamine (EDA) and glutaraldehyde (GA). The optimal concentration of GA for immobilization was 5%. The amount of loaded urease reached 157 µg/mg mat, exhibiting 54% of the free urease activity. The surface chemistry of as-spun and chemically treated fibers was examined with Fourier transform infrared (FTIR) spectroscopy. Field emission scanning electron microscopy (FESEM) was used to study the morphology and diameter of the pristine, chemically treated, and urease-immobilized microfibrous mats. Immobilized urease showed increased temperature for maximum activity (from 37 to 50 °C for free and immobilized urease, respectively) and improved storage stability (20 days). The immobilized urease was also less sensitive to the changes in pH, especially in acid conditions. In addition, nearly 70% of initial activity of the immobilized urease was retained after 15 cycles of reuse, which proved the applicability of the electrospun fibers as successful enzyme carriers. |
| first_indexed | 2024-03-05T19:39:08Z |
| format | Article |
| id | utm.eprints-55933 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:39:08Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | utm.eprints-559332017-08-25T08:52:24Z http://eprints.utm.my/55933/ Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis Daneshfar, Aref Matsuura, Takeshi Emadzadeh, Daryoush Pahlevani, Zahra Ismail, Ahmad Fauzi TP Chemical technology Electrospinning was used to fabricate beadless microfibrous polyacrylonitrile (ePAN) mats with an average fiber diameter of 1448 ± 380 nm from a 10 wt.% PAN in dimethylformamide (DMF) dope solution at applied voltage of 18 kV and 20 cm fiber collection distance. Urease (EC 3.5.1.5) was then covalently immobilized on dispersed microfibrous ePAN mats following the chemical treatment of fibers with ethylenediamine (EDA) and glutaraldehyde (GA). The optimal concentration of GA for immobilization was 5%. The amount of loaded urease reached 157 µg/mg mat, exhibiting 54% of the free urease activity. The surface chemistry of as-spun and chemically treated fibers was examined with Fourier transform infrared (FTIR) spectroscopy. Field emission scanning electron microscopy (FESEM) was used to study the morphology and diameter of the pristine, chemically treated, and urease-immobilized microfibrous mats. Immobilized urease showed increased temperature for maximum activity (from 37 to 50 °C for free and immobilized urease, respectively) and improved storage stability (20 days). The immobilized urease was also less sensitive to the changes in pH, especially in acid conditions. In addition, nearly 70% of initial activity of the immobilized urease was retained after 15 cycles of reuse, which proved the applicability of the electrospun fibers as successful enzyme carriers. Elsevier 2015-02 Article PeerReviewed Daneshfar, Aref and Matsuura, Takeshi and Emadzadeh, Daryoush and Pahlevani, Zahra and Ismail, Ahmad Fauzi (2015) Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis. Reactive and Functional Polymers, 87 . pp. 37-45. ISSN 1381-5148 http://dx.doi.org/10.1016/j.reactfunctpolym.2014.12.004 DOI:10.1016/j.reactfunctpolym.2014.12.004 |
| spellingShingle | TP Chemical technology Daneshfar, Aref Matsuura, Takeshi Emadzadeh, Daryoush Pahlevani, Zahra Ismail, Ahmad Fauzi Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis |
| title | Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis |
| title_full | Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis |
| title_fullStr | Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis |
| title_full_unstemmed | Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis |
| title_short | Urease-carrying electrospun polyacrylonitrile mat for urea hydrolysis |
| title_sort | urease carrying electrospun polyacrylonitrile mat for urea hydrolysis |
| topic | TP Chemical technology |
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