An S188V Mutation Alters Substrate Specificity of Non- Stereospecific alpha-Haloalkanoic Acid Dehalogenase E (DehE)
The non-stereospecific α-haloalkanoic acid dehalogenase E (DehE) degrades many halogenated compounds but is ineffective against β-halogenated compounds such as 3-chloropropionic acid (3CP). Using molecular dynamics (MD) simulations and site-directed mutagenesis we show here that introducing the muta...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit UTM Press
2015
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| Subjects: | |
| Online Access: | http://eprints.utm.my/57794/1/RoswaniraAbdulWahab2015_AnS188VMutationAltersSubstrateSpecificity.pdf |