Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli
Succinic acid is considers to be a platform chemical with divergent applications as a precursor for syntheses of commodity and specialty chemicals. Its biobased production could be a green technology when produced by microbial fermentation using Escherichia coli as a chassis host. Metabolic eng...
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| Format: | Article |
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2015
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| _version_ | 1796860535258480640 |
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| author | Mienda, B. S. Shamsir, M. S. |
| author_facet | Mienda, B. S. Shamsir, M. S. |
| author_sort | Mienda, B. S. |
| collection | ePrints |
| description | Succinic acid is considers to be a platform chemical with
divergent applications as a precursor for syntheses of
commodity and specialty chemicals. Its biobased production
could be a green technology when produced by
microbial fermentation using Escherichia coli as a chassis
host. Metabolic engineering for increased production
of succinic acid using glucose as substrate is beset with
limitation of NADH availability under anaerobic
conditions in E. coli. In the present work, we used the
most recent genome scale metabolic model of E. coli
iJ01366 (Orth et al. 2011) to predict metabolic gene
knockout of mdh/b3236 with the aim of increasing
NADH availability using the glucose substrate. |
| first_indexed | 2024-03-05T19:42:49Z |
| format | Article |
| id | utm.eprints-58572 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:42:49Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | utm.eprints-585722021-09-14T08:40:15Z http://eprints.utm.my/58572/ Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli Mienda, B. S. Shamsir, M. S. Q Science (General) Succinic acid is considers to be a platform chemical with divergent applications as a precursor for syntheses of commodity and specialty chemicals. Its biobased production could be a green technology when produced by microbial fermentation using Escherichia coli as a chassis host. Metabolic engineering for increased production of succinic acid using glucose as substrate is beset with limitation of NADH availability under anaerobic conditions in E. coli. In the present work, we used the most recent genome scale metabolic model of E. coli iJ01366 (Orth et al. 2011) to predict metabolic gene knockout of mdh/b3236 with the aim of increasing NADH availability using the glucose substrate. 2015 Article PeerReviewed Mienda, B. S. and Shamsir, M. S. (2015) Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli. Journal Of Biomolecular Structure & Dymics, 33 . pp. 98-100. ISSN 7351-933 http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:99480 |
| spellingShingle | Q Science (General) Mienda, B. S. Shamsir, M. S. Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli |
| title | Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli |
| title_full | Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli |
| title_fullStr | Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli |
| title_full_unstemmed | Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli |
| title_short | Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli |
| title_sort | model aided anaerobic metabolic gene knockout of malate dehydrogenase mdh gene predicts increased succinate production in escherichia coli |
| topic | Q Science (General) |
| work_keys_str_mv | AT miendabs modelaidedanaerobicmetabolicgeneknockoutofmalatedehydrogenasemdhgenepredictsincreasedsuccinateproductioninescherichiacoli AT shamsirms modelaidedanaerobicmetabolicgeneknockoutofmalatedehydrogenasemdhgenepredictsincreasedsuccinateproductioninescherichiacoli |