Overexpression, purification and characterization of aspergillus niger beta-glucosidase in pichia pastoris
This study describes the expression of ß-glucosidase (BglA) from Aspergillus niger in Pichia pastoris, a methylotrophic yeast strain, under the regulation of an alcohol oxidase promoter. The heterologous expression of BglA was optimized in a shake flask. Optimal conditions were achieved using an ini...
| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Malaysian Society of Applied Biology
2015
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| Subjects: |