Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)

The effects of Auranofin (AF) on protein expression and protein oxidation in A2780 cancer cells were investigated through a strategy based on simultaneous expression proteomics and redox proteomics determinations. Bioinformatics analysis of the proteomics data supports the view that the most critica...

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Main Authors: Giovanni Chiappetta, Tania Gamberi, Fiorella Faienza, Xhesika Limaj, Salvatore Rizza, Luigi Messori, Giuseppe Filomeni, Alessandra Modesti, Joelle Vinh
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:Redox Biology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2213231722000660
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author Giovanni Chiappetta
Tania Gamberi
Fiorella Faienza
Xhesika Limaj
Salvatore Rizza
Luigi Messori
Giuseppe Filomeni
Alessandra Modesti
Joelle Vinh
author_facet Giovanni Chiappetta
Tania Gamberi
Fiorella Faienza
Xhesika Limaj
Salvatore Rizza
Luigi Messori
Giuseppe Filomeni
Alessandra Modesti
Joelle Vinh
author_sort Giovanni Chiappetta
collection DOAJ
description The effects of Auranofin (AF) on protein expression and protein oxidation in A2780 cancer cells were investigated through a strategy based on simultaneous expression proteomics and redox proteomics determinations. Bioinformatics analysis of the proteomics data supports the view that the most critical cellular changes elicited by AF treatment consist of thioredoxin reductase inhibition, alteration of the cell redox state, impairment of the mitochondrial functions, metabolic changes associated with conversion to a glycolytic phenotype, induction of ER stress. The occurrence of the above cellular changes was extensively validated by performing direct biochemical assays. Our data are consistent with the concept that AF produces its effects through a multitarget mechanism that mainly affects the redox metabolism and the mitochondrial functions and results into severe ER stress. Results are discussed in the context of the current mechanistic knowledge existing on AF.
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spelling doaj.art-7eb4262de80b4b418a84168123ed4a6c2022-12-22T00:38:43ZengElsevierRedox Biology2213-23172022-06-0152102294Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)Giovanni Chiappetta0Tania Gamberi1Fiorella Faienza2Xhesika Limaj3Salvatore Rizza4Luigi Messori5Giuseppe Filomeni6Alessandra Modesti7Joelle Vinh8Biological Mass Spectrometry and Proteomics Group, SMBP, PDC CNRS UMR, 8249, ESPCI Paris, Université PSL, 10 rue Vauquelin, 75005, Paris, France; Corresponding author.Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale G.B. Morgagni 50, 50134, Florence, Italy; Corresponding author.Department of Biology, University of Rome Tor Vergata, Rome, ItalyBiological Mass Spectrometry and Proteomics Group, SMBP, PDC CNRS UMR, 8249, ESPCI Paris, Université PSL, 10 rue Vauquelin, 75005, Paris, FranceRedox Signaling and Oxidative Stress Group, Danish Cancer Society Research Center, Copenhagen, DenmarkMetmed Lab, Department of Chemistry, University of Florence, via della lastruccia 3, 50019, Sesto Fiorentino, ItalyDepartment of Biology, University of Rome Tor Vergata, Rome, Italy; Redox Signaling and Oxidative Stress Group, Danish Cancer Society Research Center, Copenhagen, Denmark; Center for Healthy Aging, University of Copenhagen, DenmarkDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale G.B. Morgagni 50, 50134, Florence, ItalyBiological Mass Spectrometry and Proteomics Group, SMBP, PDC CNRS UMR, 8249, ESPCI Paris, Université PSL, 10 rue Vauquelin, 75005, Paris, FranceThe effects of Auranofin (AF) on protein expression and protein oxidation in A2780 cancer cells were investigated through a strategy based on simultaneous expression proteomics and redox proteomics determinations. Bioinformatics analysis of the proteomics data supports the view that the most critical cellular changes elicited by AF treatment consist of thioredoxin reductase inhibition, alteration of the cell redox state, impairment of the mitochondrial functions, metabolic changes associated with conversion to a glycolytic phenotype, induction of ER stress. The occurrence of the above cellular changes was extensively validated by performing direct biochemical assays. Our data are consistent with the concept that AF produces its effects through a multitarget mechanism that mainly affects the redox metabolism and the mitochondrial functions and results into severe ER stress. Results are discussed in the context of the current mechanistic knowledge existing on AF.http://www.sciencedirect.com/science/article/pii/S2213231722000660Redox proteomicsCysteineAuranofinGold drugsOvarian cancer
spellingShingle Giovanni Chiappetta
Tania Gamberi
Fiorella Faienza
Xhesika Limaj
Salvatore Rizza
Luigi Messori
Giuseppe Filomeni
Alessandra Modesti
Joelle Vinh
Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)
Redox Biology
Redox proteomics
Cysteine
Auranofin
Gold drugs
Ovarian cancer
title Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)
title_full Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)
title_fullStr Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)
title_full_unstemmed Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)
title_short Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780)
title_sort redox proteome analysis of auranofin exposed ovarian cancer cells a2780
topic Redox proteomics
Cysteine
Auranofin
Gold drugs
Ovarian cancer
url http://www.sciencedirect.com/science/article/pii/S2213231722000660
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