Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells

Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells

Summary: Immune synapse (IS) formation determines T cell antitumor activity. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging. We describe steps for CAR T cel...

Bibliográfalaš dieđut
Váldodahkkit:	Jorge Ibanez, Nikhil Hebbar, Unmesha Thanekar, Zhongzhen Yi, Haley Houke, Meghan Ward, Chris Nevitt, Liqing Tian, Stephen C. Mack, Heather Sheppard, Jason Chiang, M. Paulina Velasquez, Giedre Krenciute
Materiálatiipa:	Artihkal
Giella:	English
Almmustuhtton:	Elsevier 2024-12-01
Ráidu:	STAR Protocols
Fáttát:	cancer immunology microscopy
Liŋkkat:	http://www.sciencedirect.com/science/article/pii/S2666166724005872

Geahča maid

289 Defining the T cell functional attributes of effective CAR T cell donors
Dahkki: Giedre Krenciute, et al.
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Optimizing EphA2-CAR T Cells for the Adoptive Immunotherapy of Glioma
Dahkki: Zhongzhen Yi, et al.
Almmustuhtton: (2018-06-01)

Next Generation CAR T Cells for the Immunotherapy of High-Grade Glioma
Dahkki: Christopher T. Petersen, et al.
Almmustuhtton: (2019-02-01)

CAR T cells redirected to cell surface GRP78 display robust anti-acute myeloid leukemia activity and do not target hematopoietic progenitor cells
Dahkki: Nikhil Hebbar, et al.
Almmustuhtton: (2022-01-01)

Improving the anti-acute myeloid leukemia activity of CD123-specific engager T cells by MyD88 and CD40 costimulation
Dahkki: Abishek Vaidya, et al.
Almmustuhtton: (2022-07-01)

263 Unveiling the role of macrophages in CAR T-cell induced remodeling of the brain tumor immune microenvironment: implications for anti-glioma adoptive immunotherapy
Dahkki: Paul Thomas, et al.
Almmustuhtton: (2023-11-01)

CD47 expression is critical for CAR T-cell survival in vivo
Dahkki: Stephen Gottschalk, et al.
Almmustuhtton: (2023-03-01)

Hypoxia-inducible CAR expression: An answer to the on-target/off-tumor dilemma?
Dahkki: Brooke Prinzing, et al.
Almmustuhtton: (2021-04-01)

T-Cell Immunotherapy for Pediatric High-Grade Gliomas: New Insights to Overcoming Therapeutic Challenges
Dahkki: Dalia Haydar, et al.
Almmustuhtton: (2021-10-01)

IL-18R supported CAR T cells targeting oncofetal tenascin C for the immunotherapy of pediatric sarcoma and brain tumors
Dahkki: Jessica Wagner, et al.
Almmustuhtton: (2024-11-01)

MyD88/CD40 signaling retains CAR T cells in a less differentiated state
Dahkki: Brooke Prinzing, et al.
Almmustuhtton: (2020-11-01)

Tyrosine kinase signaling-independent MET-targeting with CAR-T cells
Dahkki: Anna Qin, et al.
Almmustuhtton: (2023-10-01)

T cell-dendritic cell immunological synapses.
Dahkki: Dustin, M, et al.
Almmustuhtton: (2006)

250 Manipulating T cell epigenetic programs to improve persistence against group 3 pediatric medulloblastoma
Dahkki: Benjamin Youngblood, et al.
Almmustuhtton: (2023-11-01)

Deep-learning-based three-dimensional label-free tracking and analysis of immunological synapses of CAR-T cells
Dahkki: Moosung Lee, et al.
Almmustuhtton: (2020-12-01)

T Cell Polarization at the Virological Synapse
Dahkki: Clare Jolly
Almmustuhtton: (2010-05-01)

Harnessing T Cells to Target Pediatric Acute Myeloid Leukemia: CARs, BiTEs, and Beyond
Dahkki: Rebecca Epperly, et al.
Almmustuhtton: (2020-02-01)

Nanoscale CAR Organization at the Immune Synapse Correlates with CAR-T Effector Functions
Dahkki: Julia Sajman, et al.
Almmustuhtton: (2023-09-01)

Visualization of cell-cell interaction contacts-synapses and kinapses.
Dahkki: Dustin, M
Almmustuhtton: (2008)

Visualization of Cell-Cell Interaction Contacts: Synapses and Kinapses.
Dahkki: Dustin, M
Almmustuhtton: (2011)

CELL TO CELL COMMUNICATION IN GLIOBLASTOMA PROGRESSION: INTRATUMORAL SYNAPSES?
Dahkki: María Losada-Pérez, et al.
Almmustuhtton: (2023-10-01)

136 Targeting MET with chimeric antigen receptor T cells in hepatocellular carcinoma
Dahkki: Giedre Krenciute, et al.
Almmustuhtton: (2020-11-01)

Impact of the immunological synapse on T cell signaling.
Dahkki: Dustin, M
Almmustuhtton: (2006)

Cell-Type-Specific Neuroproteomics of Synapses
Dahkki: Yun Young Yim, et al.
Almmustuhtton: (2023-06-01)

The dendritic cell side of the immunological synapse
Dahkki: Verboogen Danielle R.J., et al.
Almmustuhtton: (2016-02-01)

Chimeric antigen receptor (CAR)-modified natural killer cell-based immunotherapy and immunological synapse formation in cancer and HIV
Dahkki: Dongfang Liu, et al.
Almmustuhtton: (2017-05-01)

CAR and TCR form individual signaling synapses and do not cross-activate, however, can co-operate in T cell activation
Dahkki: Markus Barden, et al.
Almmustuhtton: (2023-02-01)

The dendritic cell synapse: a life dedicated to T cell activation
Dahkki: Federica eBenvenuti
Almmustuhtton: (2016-03-01)

Universal CARs, universal T cells, and universal CAR T cells
Dahkki: Juanjuan Zhao, et al.
Almmustuhtton: (2018-11-01)

Route of 41BB/41BBL Costimulation Determines Effector Function of B7-H3-CAR.CD28ζ T Cells
Dahkki: Phuong Nguyen, et al.
Almmustuhtton: (2020-09-01)

Not All T Cell Synapses Are Built the Same Way
Dahkki: Kumari, Sudha, et al.
Almmustuhtton: (2020)

Regulatory T cell immunological synapse in rheumatoid arthritis
Dahkki: Dustin, M
Almmustuhtton: (2016)

Functional anatomy of T cell activation and synapse formation.
Dahkki: Fooksman, DR, et al.
Almmustuhtton: (2010)

T-cell activation through immunological synapses and kinapses.
Dahkki: Dustin, M
Almmustuhtton: (2008)

Not all T cell synapses are built the same way
Dahkki: Kumari, S, et al.
Almmustuhtton: (2019)

The integration of signalling and the spatial organization of the T cell synapse
Dahkki: Jeremie eRossy, et al.
Almmustuhtton: (2012-11-01)

302 Generation of immune-evasive allogeneic CAR T cells by inactivation of the HLA transcriptional regulator RFX5 and disruption of the immune synapse
Dahkki: Barbra Sasu, et al.
Almmustuhtton: (2023-11-01)

Erratum to: Chimeric antigen receptor (CAR)-modified natural killer cell-based immunotherapy and immunological synapse formation in cancer and HIV
Dahkki: Dongfang Liu, et al.
Almmustuhtton: (2017-06-01)

Interplay of periglomerular and granule cell inhibitory synapses on mitral cell spiking
Dahkki: Roque Antonio C, et al.
Almmustuhtton: (2011-07-01)

Prediction of severe CRS and determination of biomarkers in B cell-acute lymphoblastic leukemia treated with CAR-T cells
Dahkki: Zhenyu Wei, et al.
Almmustuhtton: (2023-10-01)