Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies

We have previously reported that the leaf extract of <i>Albizia anthelmintica</i> exhibited substantial antioxidant, anti-inflammatory, analgesic, and antipyretic properties in vivo. We also comprehensively characterized the active phytoconstituents and found several flavonoids and gallo...

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Main Authors: Mohamed Nabil, Mohamed A. El Raey, Walied Abdo, Mohamed A. O. Abdelfattah, Assem M. El-Shazly, Mansour Sobeh, Mona F. Mahmoud
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Antioxidants
Subjects:
Online Access:https://www.mdpi.com/2076-3921/10/2/176
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author Mohamed Nabil
Mohamed A. El Raey
Walied Abdo
Mohamed A. O. Abdelfattah
Assem M. El-Shazly
Mansour Sobeh
Mona F. Mahmoud
author_facet Mohamed Nabil
Mohamed A. El Raey
Walied Abdo
Mohamed A. O. Abdelfattah
Assem M. El-Shazly
Mansour Sobeh
Mona F. Mahmoud
author_sort Mohamed Nabil
collection DOAJ
description We have previously reported that the leaf extract of <i>Albizia anthelmintica</i> exhibited substantial antioxidant, anti-inflammatory, analgesic, and antipyretic properties in vivo. We also comprehensively characterized the active phytoconstituents and found several flavonoids and galloyl glucosides derivatives. In the current work, we explored the gastroprotective effects of the leaf extract in an indomethacin-induced ulcer model and the mechanisms involved. The rats being pretreated with the tested extract (100 and 200 mg kg<sup>−1</sup>) significantly prevented gastric lesions by 87.4% and 92.3%, respectively, and they had no structural derangements in the gastric mucosa. The extract significantly reduced the elevated levels of IKκB, NF-κB, TNF-α, IL-6, iNOS, and lipid peroxidation; increased the reduced level of glutathione peroxidase (GPx) activity; and reduced glutathione (GSH) in the indomethacin-induced ulcer model. The protective activities of the extract were similar in most aspects to those exerted by the known anti-ulcer drug famotidine. These activities might be attributed to the anti-inflammatory and antioxidant activities, and the reduction of iNOS levels. In conclusion, <i>Albizia anthelmintica</i> is a potential candidate for management of gastric ulcers with antioxidant properties.
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spelling doaj.art-93004547c93b490aa571c62acef8bd7e2023-12-03T14:45:09ZengMDPI AGAntioxidants2076-39212021-01-0110217610.3390/antiox10020176Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo StudiesMohamed Nabil0Mohamed A. El Raey1Walied Abdo2Mohamed A. O. Abdelfattah3Assem M. El-Shazly4Mansour Sobeh5Mona F. Mahmoud6Pharmaceutical and Fermentation Industries Development Center (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab 21934, EgyptDepartment of Phytochemistry and Plant Systematics, Pharmaceutical Division, National Research Centre, Dokki, Cairo 12622, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El Sheikh 33516, EgyptCollege of Engineering and Technology, American University of the Middle East, KuwaitDepartment of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptAgroBioSciences Research Department, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, Ben-Guerir 43150, MoroccoDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptWe have previously reported that the leaf extract of <i>Albizia anthelmintica</i> exhibited substantial antioxidant, anti-inflammatory, analgesic, and antipyretic properties in vivo. We also comprehensively characterized the active phytoconstituents and found several flavonoids and galloyl glucosides derivatives. In the current work, we explored the gastroprotective effects of the leaf extract in an indomethacin-induced ulcer model and the mechanisms involved. The rats being pretreated with the tested extract (100 and 200 mg kg<sup>−1</sup>) significantly prevented gastric lesions by 87.4% and 92.3%, respectively, and they had no structural derangements in the gastric mucosa. The extract significantly reduced the elevated levels of IKκB, NF-κB, TNF-α, IL-6, iNOS, and lipid peroxidation; increased the reduced level of glutathione peroxidase (GPx) activity; and reduced glutathione (GSH) in the indomethacin-induced ulcer model. The protective activities of the extract were similar in most aspects to those exerted by the known anti-ulcer drug famotidine. These activities might be attributed to the anti-inflammatory and antioxidant activities, and the reduction of iNOS levels. In conclusion, <i>Albizia anthelmintica</i> is a potential candidate for management of gastric ulcers with antioxidant properties.https://www.mdpi.com/2076-3921/10/2/176<i>Albizia anthelmintica</i>gastric ulcerindomethacinflavonoids
spellingShingle Mohamed Nabil
Mohamed A. El Raey
Walied Abdo
Mohamed A. O. Abdelfattah
Assem M. El-Shazly
Mansour Sobeh
Mona F. Mahmoud
Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
Antioxidants
<i>Albizia anthelmintica</i>
gastric ulcer
indomethacin
flavonoids
title Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
title_full Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
title_fullStr Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
title_full_unstemmed Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
title_short Gastro-Protective Effects of <i>Albizia anthelmintica</i> Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
title_sort gastro protective effects of i albizia anthelmintica i leaf extract on indomethacin induced gastric ulcer in wistar rats in silico and in vivo studies
topic <i>Albizia anthelmintica</i>
gastric ulcer
indomethacin
flavonoids
url https://www.mdpi.com/2076-3921/10/2/176
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