Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Summary: Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene’s effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 tech...

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Sonraí bibleagrafaíochta
Príomhchruthaitheoirí: Anne Huber, Christine Dijkstra, Matthias Ernst, Moritz F. Eissmann
Formáid: Alt
Teanga:English
Foilsithe / Cruthaithe: Elsevier 2023-03-01
Sraith:STAR Protocols
Ábhair:
Rochtain ar líne:http://www.sciencedirect.com/science/article/pii/S2666166723000345