Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Summary: Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene’s effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 tech...

詳細記述

書誌詳細
主要な著者: Anne Huber, Christine Dijkstra, Matthias Ernst, Moritz F. Eissmann
フォーマット: 論文
言語:English
出版事項: Elsevier 2023-03-01
シリーズ:STAR Protocols
主題:
オンライン・アクセス:http://www.sciencedirect.com/science/article/pii/S2666166723000345