Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

Summary: Mpox is caused by a zoonotic virus belonging to the Orthopoxvirus genus and the Poxviridae family. In this study, we develop a recombinase polymerase amplification (RPA)-coupled CRISPR-Cas12a detection assay for the mpox virus. We design and test a series of CRISPR-derived RNAs(crRNAs) targ...

Повний опис

Бібліографічні деталі
Автори: Fei Zhao, Yamei Hu, Zhangling Fan, Baoying Huang, Liang Wei, Yu Xie, Yu Huang, Shan Mei, Liming Wang, Lingwa Wang, Bin Ai, Jugao Fang, Chen Liang, Fengwen Xu, Wenjie Tan, Fei Guo
Формат: Стаття
Мова:English
Опубліковано: Elsevier 2023-10-01
Серія:Cell Reports: Methods
Предмети:
CP: Biotechnology
CP: Microbiology
Онлайн доступ:http://www.sciencedirect.com/science/article/pii/S2667237523002849

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http://www.sciencedirect.com/science/article/pii/S2667237523002849

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