Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

Summary: Mpox is caused by a zoonotic virus belonging to the Orthopoxvirus genus and the Poxviridae family. In this study, we develop a recombinase polymerase amplification (RPA)-coupled CRISPR-Cas12a detection assay for the mpox virus. We design and test a series of CRISPR-derived RNAs(crRNAs) targ...

Descripció completa

Dades bibliogràfiques
Autors principals: Fei Zhao, Yamei Hu, Zhangling Fan, Baoying Huang, Liang Wei, Yu Xie, Yu Huang, Shan Mei, Liming Wang, Lingwa Wang, Bin Ai, Jugao Fang, Chen Liang, Fengwen Xu, Wenjie Tan, Fei Guo
Format: Article
Idioma:English
Publicat: Elsevier 2023-10-01
Col·lecció:Cell Reports: Methods
Matèries:
CP: Biotechnology
CP: Microbiology
Accés en línia:http://www.sciencedirect.com/science/article/pii/S2667237523002849

Internet

http://www.sciencedirect.com/science/article/pii/S2667237523002849

Ítems similars