Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation
BackgroundThe peroxiredoxin family, a crucial regulator of redox reactions, is strongly associated with various tumorigenesis. However, the role of peroxiredoxin4 (PRDX4) in colon adenocarcinoma (COAD) remains poorly understood.MethodsMulticenter databases, including GEPIA, HPA, UALCAN, cBioPortal,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1136738/full |
| _version_ | 1827994309339643904 |
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| author | He Zhou He Zhou He Zhou Lifa Li Lifa Li Jia Chen Jia Chen Songlin Hou Songlin Hou Tong Zhou Tong Zhou Yongfu Xiong Yongfu Xiong |
| author_facet | He Zhou He Zhou He Zhou Lifa Li Lifa Li Jia Chen Jia Chen Songlin Hou Songlin Hou Tong Zhou Tong Zhou Yongfu Xiong Yongfu Xiong |
| author_sort | He Zhou |
| collection | DOAJ |
| description | BackgroundThe peroxiredoxin family, a crucial regulator of redox reactions, is strongly associated with various tumorigenesis. However, the role of peroxiredoxin4 (PRDX4) in colon adenocarcinoma (COAD) remains poorly understood.MethodsMulticenter databases, including GEPIA, HPA, UALCAN, cBioPortal, cancerSEA, STRING, CCLE, and LinkedOmics, comprehensively analyzed transcriptional expression, prognostic value, genetic alterations, signaling pathways, and associated genes of the PRDXs in COAD patients. Colony formation, transwell, flow cytometry, sphere formation, and xenograft assays were performed to validate further in vitro and in vivo.ResultsMembers of the PRDX family were differentially expressed in COAD, with each member showing varying degrees of genetic alterations. Intriguingly, only PRDX4 significantly correlated with COAD prognosis and stage. The single-cell sequencing suggested that PRDX4 is positively correlated with proliferation, apoptosis, and invasion, whereas negatively correlated with stemness. Moreover, PRDX4 involved in a series of critical biological processes, such as cell growth. Furthermore, in vivo and in vitro analyses indicated that knocking down PRDX4 inhibits the proliferation and invasion of HCT116 cells while promoting apoptosis and stemness.ConclusionsWe identified PRDX4 expression as a novel potential prognostic marker in COAD. |
| first_indexed | 2024-04-10T04:37:56Z |
| format | Article |
| id | doaj.art-d1347453cfde4a7ebe59034beddc6f91 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T04:37:56Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-d1347453cfde4a7ebe59034beddc6f912023-03-09T13:08:02ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-03-011310.3389/fonc.2023.11367381136738Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validationHe Zhou0He Zhou1He Zhou2Lifa Li3Lifa Li4Jia Chen5Jia Chen6Songlin Hou7Songlin Hou8Tong Zhou9Tong Zhou10Yongfu Xiong11Yongfu Xiong12The Second Department of Gastrointestinal Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaInstitute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, ChinaLaboratory of Cancer Biology Department of Oncology, University of Oxford, Oxford, United KingdomThe Second Department of Gastrointestinal Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaInstitute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, ChinaThe Second Department of Gastrointestinal Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaInstitute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, ChinaThe Second Department of Gastrointestinal Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaInstitute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, ChinaThe Second Department of Gastrointestinal Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaInstitute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, ChinaInstitute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, ChinaThe Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaBackgroundThe peroxiredoxin family, a crucial regulator of redox reactions, is strongly associated with various tumorigenesis. However, the role of peroxiredoxin4 (PRDX4) in colon adenocarcinoma (COAD) remains poorly understood.MethodsMulticenter databases, including GEPIA, HPA, UALCAN, cBioPortal, cancerSEA, STRING, CCLE, and LinkedOmics, comprehensively analyzed transcriptional expression, prognostic value, genetic alterations, signaling pathways, and associated genes of the PRDXs in COAD patients. Colony formation, transwell, flow cytometry, sphere formation, and xenograft assays were performed to validate further in vitro and in vivo.ResultsMembers of the PRDX family were differentially expressed in COAD, with each member showing varying degrees of genetic alterations. Intriguingly, only PRDX4 significantly correlated with COAD prognosis and stage. The single-cell sequencing suggested that PRDX4 is positively correlated with proliferation, apoptosis, and invasion, whereas negatively correlated with stemness. Moreover, PRDX4 involved in a series of critical biological processes, such as cell growth. Furthermore, in vivo and in vitro analyses indicated that knocking down PRDX4 inhibits the proliferation and invasion of HCT116 cells while promoting apoptosis and stemness.ConclusionsWe identified PRDX4 expression as a novel potential prognostic marker in COAD.https://www.frontiersin.org/articles/10.3389/fonc.2023.1136738/fullperoxiredoxin familybioinformatics analysisbiomarkerprognosiscolorectal cancer |
| spellingShingle | He Zhou He Zhou He Zhou Lifa Li Lifa Li Jia Chen Jia Chen Songlin Hou Songlin Hou Tong Zhou Tong Zhou Yongfu Xiong Yongfu Xiong Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation Frontiers in Oncology peroxiredoxin family bioinformatics analysis biomarker prognosis colorectal cancer |
| title | Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation |
| title_full | Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation |
| title_fullStr | Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation |
| title_full_unstemmed | Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation |
| title_short | Expression and prognostic value of PRDX family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation |
| title_sort | expression and prognostic value of prdx family in colon adenocarcinoma by integrating comprehensive analysis and in vitro and in vivo validation |
| topic | peroxiredoxin family bioinformatics analysis biomarker prognosis colorectal cancer |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1136738/full |
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