Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering
The physicochemical context in which molecules are presented at the surfaces of microbes has tremendous implications for the immune response to vaccination. The spacing and mobility of molecules may control interactions of their receptors, influencing immune cell activation, pathogen uptake, and ant...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Institute of Electrical and Electronics Engineers
2010
|
| Online Access: | http://hdl.handle.net/1721.1/58904 |
| _version_ | 1811074846983979008 |
|---|---|
| author | Chaparro, Jose P. Bershteyn, Anna Riley, E. B. Yao, R. S. Zachariah, Roshini Sarah Irvine, Darrell J. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Chaparro, Jose P. Bershteyn, Anna Riley, E. B. Yao, R. S. Zachariah, Roshini Sarah Irvine, Darrell J. |
| author_sort | Chaparro, Jose P. |
| collection | MIT |
| description | The physicochemical context in which molecules are presented at the surfaces of microbes has tremendous implications for the immune response to vaccination. The spacing and mobility of molecules may control interactions of their receptors, influencing immune cell activation, pathogen uptake, and antigen processing. The chemical environment of antigens also influences the specificity of the humoral immune response, because antibodies recognize antigen in its three-dimensional shape and context. Finally, physical properties of antigen, such as diameter, impact immune response on both a cellular and tissue level. We have constructed ldquosynthetic pathogensrdquo consisting of a biodegradable core polymer coated by a lipid shell to mimic a bilayer-enveloped pathogen. Synthesized in an oil-in-water emulsion, these particles have an average diameter on the order of either 100 nm, mimicking a lipid-enveloped viral pathogen, or 1 micron, mimicking a bacterial pathogen. CryoEM reveals self-assembled lipid layers at the particle surface. With tunable chemical and physical properties, these particles can be used to study the importance of specific properties of biomaterials when used in vaccination. Because all components are biodegradable, the particles may provide a clinically applicable way of implementing structural features of microbes in synthetic vaccines. |
| first_indexed | 2024-09-23T09:56:22Z |
| format | Article |
| id | mit-1721.1/58904 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:56:22Z |
| publishDate | 2010 |
| publisher | Institute of Electrical and Electronics Engineers |
| record_format | dspace |
| spelling | mit-1721.1/589042022-09-30T17:50:32Z Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering Chaparro, Jose P. Bershteyn, Anna Riley, E. B. Yao, R. S. Zachariah, Roshini Sarah Irvine, Darrell J. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Materials Science and Engineering Irvine, Darrell J. Chaparro, Jose P. Bershteyn, Anna Riley, E. B. Yao, R. S. Zachariah, Roshini Sarah Irvine, Darrell J. The physicochemical context in which molecules are presented at the surfaces of microbes has tremendous implications for the immune response to vaccination. The spacing and mobility of molecules may control interactions of their receptors, influencing immune cell activation, pathogen uptake, and antigen processing. The chemical environment of antigens also influences the specificity of the humoral immune response, because antibodies recognize antigen in its three-dimensional shape and context. Finally, physical properties of antigen, such as diameter, impact immune response on both a cellular and tissue level. We have constructed ldquosynthetic pathogensrdquo consisting of a biodegradable core polymer coated by a lipid shell to mimic a bilayer-enveloped pathogen. Synthesized in an oil-in-water emulsion, these particles have an average diameter on the order of either 100 nm, mimicking a lipid-enveloped viral pathogen, or 1 micron, mimicking a bacterial pathogen. CryoEM reveals self-assembled lipid layers at the particle surface. With tunable chemical and physical properties, these particles can be used to study the importance of specific properties of biomaterials when used in vaccination. Because all components are biodegradable, the particles may provide a clinically applicable way of implementing structural features of microbes in synthetic vaccines. Paul & Daisy Soros Fellowships for New Americans (New York, N.Y.) Hertz Foundation Human Frontier Science Program (Strasbourg, France) Massachusetts Institute of Technology. Undergraduate Research Opportunities Program United States. Defense Advanced Research Projects Agency National Science Foundation (U.S.) National Institutes of Health (U.S.) National Science Foundation (U.S.). Graduate Research Fellowship 2010-10-06T15:52:36Z 2010-10-06T15:52:36Z 2009-05 2009-04 Article http://purl.org/eprint/type/JournalArticle 978-1-4244-4362-8 INSPEC Accession Number: 10666520 http://hdl.handle.net/1721.1/58904 Bershteyn, A. et al. “Lipid-coated biodegradable particles as “synthetic pathogens” for vaccine engineering.” Bioengineering Conference, 2009 IEEE 35th Annual Northeast. 2009. 1-2. ©2009 Institute of Electrical and Electronics Engineers. en_US http://dx.doi.org/10.1109/NEBC.2009.4967679 2009 IEEE 35th Annual Northeast Bioengineering Conference Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Institute of Electrical and Electronics Engineers IEEE |
| spellingShingle | Chaparro, Jose P. Bershteyn, Anna Riley, E. B. Yao, R. S. Zachariah, Roshini Sarah Irvine, Darrell J. Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering |
| title | Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering |
| title_full | Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering |
| title_fullStr | Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering |
| title_full_unstemmed | Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering |
| title_short | Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering |
| title_sort | lipid coated biodegradable particles as synthetic pathogens for vaccine engineering |
| url | http://hdl.handle.net/1721.1/58904 |
| work_keys_str_mv | AT chaparrojosep lipidcoatedbiodegradableparticlesassyntheticpathogensforvaccineengineering AT bershteynanna lipidcoatedbiodegradableparticlesassyntheticpathogensforvaccineengineering AT rileyeb lipidcoatedbiodegradableparticlesassyntheticpathogensforvaccineengineering AT yaors lipidcoatedbiodegradableparticlesassyntheticpathogensforvaccineengineering AT zachariahroshinisarah lipidcoatedbiodegradableparticlesassyntheticpathogensforvaccineengineering AT irvinedarrellj lipidcoatedbiodegradableparticlesassyntheticpathogensforvaccineengineering |