Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors
In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in E...
全面介绍
书目详细资料
| Main Authors: |
Bird, L,
Rada, H,
Flanagan, J,
Diprose, J,
Gilbert, R,
Owens, R |
|---|
| 格式: | Journal article
|
|---|
| 语言: | English |
|---|
| 出版: |
Humana Press Inc.
2014
|
|---|