Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors
In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in E...
Główni autorzy: | Bird, L, Rada, H, Flanagan, J, Diprose, J, Gilbert, R, Owens, R |
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Format: | Journal article |
Język: | English |
Wydane: |
Humana Press Inc.
2014
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