Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.

Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.

<p><strong>Background</strong> CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up to 16% efficiency in generating...

書誌詳細
主要な著者:	Gurumurthy, C, O'Brien, A, Quadros, R, Adams, J, Alcaide, P, Ayabe, S, Ballard, J, Batra, S, Beauchamp, M, Becker, K, Bernas, G, Brough, D, Carrillo-Salinas, F, Chan, W, Chen, H, Dawson, R, Demambro, V, D'Hont, J, Dibb, K, Eudy, J, Gan, L, Gao, J, Gonzales, A, Guntur, A, Guo, H, Harms, D, Harrington, A, Hentges, K, Humphreys, N, Imai, S, Ishii, H, Iwama, M, Jonasch, E, Karolak, M, Keavney, B, Khin, N, Konno, M, Kotani, Y, Kunihiro, Y, Lakshmanan, I, Larochelle, C, Lawrence, C, Li, L, Lindner, V, Liu, X, Lopez-Castejon, G, Loudon, A, Lowe, J, Jerome-Majewska, L, Matsusaka, T, Miura, H, Miyasaka, Y, Morpurgo, B, Motyl, K, Nabeshima, Y, Nakade, K, Nakashiba, T, Nakashima, K, Obata, Y, Ogiwara, S, Ouellet, M, Oxburgh, L, Piltz, S, Pinz, I, Ponnusamy, M, Ray, D, Redder, R, Rosen, C, Ross, N, Ruhe, M, Ryzhova, L, Salvador, A, Alam, S, Sedlacek, R, Sharma, K, Smith, C, Staes, K, Starrs, L, Sugiyama, F, Takahashi, S, Tanaka, T, Trafford, A, Uno, Y, Vanhoutte, L, Vanrockeghem, F, Willis, B, Wright, C, Yamauchi, Y, Yi, X, Yoshimi, K, Zhang, X, Zhang, Y, Ohtsuka, M, Das, S, Garry, D, Hochepied, T, Thomas, P, Parker-Thornburg, J, Adamson, A, Yoshiki, A, Schmouth, J, Golovko, A, Thompson, W, Lloyd, K, Wood, J, Cowan, M, Mashimo, T, Mizuno, S, Zhu, H, Kasparek, P, Liaw, L, Miano, J, Burgio, G
フォーマット:	Journal article
言語:	English
出版事項:	BioMed Central 2019

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