The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.

The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.

We have developed a protocol which facilitates the isolation of nuclei containing mitotic spindles from the plasmodium of Physarum polycephalum. The nuclei are isolated in a spindle stabilization buffer (SSB) consisting of -4 M glycerol, 5 mM EGTA, 10 mM MgCl2, 10 mM PIPES and 0.1% (v/v) Triton X-10...

書誌詳細
主要な著者:	Roobol, A, Paul, E, Gull, K
フォーマット:	Journal article
言語:	English
出版事項:	1984

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