The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.

The isolation of nuclei containing mitotic spindles from the plasmodium of the slime mould Physarum polycephalum.

We have developed a protocol which facilitates the isolation of nuclei containing mitotic spindles from the plasmodium of Physarum polycephalum. The nuclei are isolated in a spindle stabilization buffer (SSB) consisting of -4 M glycerol, 5 mM EGTA, 10 mM MgCl2, 10 mM PIPES and 0.1% (v/v) Triton X-10...

Полное описание

Библиографические подробности
Главные авторы:	Roobol, A, Paul, E, Gull, K
Формат:	Journal article
Язык:	English
Опубликовано:	1984

Схожие документы

Identification of tubulin isoforms in the plasmodium of Physarum polycephalum by in vitro microtubule assembly.
по: Roobol, A, и др.
Опубликовано: (1984)

ISOLATION AND CHARACTERIZATION OF THE MICROTUBULE ORGANIZING CENTER OF PHYSARUM-POLYCEPHALUM MYXAMOEBAE
по: Clayton, L, и др.
Опубликовано: (1982)

Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum.
по: Paul, E, и др.
Опубликовано: (1989)

THE MICROTUBULE-ORGANIZING CENTER OF MYXAMOEBAE OF PHYSARUM-POLYCEPHALUM
по: Havercroft, J, и др.
Опубликовано: (1981)

Microtubule nucleation by the isolated microtubule-organizing centre of Physarum polycephalum myxamoebae.
по: Roobol, A, и др.
Опубликовано: (1982)

Identification and characterization of microtubule proteins from myxamoebae of Physarum polycephalum.
по: Roobol, A, и др.
Опубликовано: (1980)

In vitro assembly of microtubule proteins from myxamoebae of Physarum polycephalum.
по: Roobol, A, и др.
Опубликовано: (1980)

Patterns of tubulin isotype synthesis and usage during mitotic spindle morphogenesis in Physarum.
по: Paul, E, и др.
Опубликовано: (1987)

Information Transfer During Food Choice in the Slime Mold Physarum polycephalum
по: Subash K. Ray, и др.
Опубликовано: (2019-03-01)

THE MOLECULAR-BIOLOGY OF THE TUBULINS OF PHYSARUM-POLYCEPHALUM
по: Dove, W, и др.
Опубликовано: (1984)

Plant hairy root cultures as plasmodium modulators of the slime mold emergent computing substrate Physarum polycephalum
по: Vincent eRicigliano, и др.
Опубликовано: (2015-07-01)

Emission and biosynthesis of volatile terpenoids from the plasmodial slime mold Physarum polycephalum
по: Xinlu Chen, и др.
Опубликовано: (2019-11-01)

ULTRASTRUCTURAL AND BIOCHEMICAL-CHARACTERIZATION OF THE CYTOSKELETON OF PHYSARUM-POLYCEPHALUM MYXAMOEBAE
по: Clayton, L, и др.
Опубликовано: (1983)

Structure and expression of an alpha-tubulin gene of Physarum polycephalum.
по: Walden, P, и др.
Опубликовано: (1989)

A correlation between in vivo and in vitro effects of the microtubule inhibitors colchicine, parbendazole and nocodazole on myxamoebae of Physarum polycephalum.
по: Quinlan, R, и др.
Опубликовано: (1981)

Biological activities and chemical compositions of slime tracks and crude exopolysaccharides isolated from plasmodia of Physarum polycephalum and Physarella oblonga
по: Tuyen T.M Huynh, и др.
Опубликовано: (2017-11-01)

Characterisation of a microtubule organising centre from Physarum polycephalum myxamoebae.
по: Havercroft, J, и др.
Опубликовано: (1981)

A comparison of tubulins from mammalian brain and Physarum polycephalum using SDS-polyacrylamide gel electrophorsis and peptide mapping.
по: Clayton, L, и др.
Опубликовано: (1980)

A first glimpse at the transcriptome of <it>Physarum polycephalum</it>
по: Meyer Sonja, и др.
Опубликовано: (2008-01-01)

EVENTS IN THE AMEBAL-PLASMODIAL TRANSITION OF PHYSARUM-POLYCEPHALUM STUDIED BY ENRICHMENT FOR COMMITTED CELLS
по: Blindt, A, и др.
Опубликовано: (1986)

Ultrastructural effects of griseofulvin on the myxomycete Physarum polycephalum. Inhibition of mitosis and the production of microtubule crystals.
по: Gull, K, и др.
Опубликовано: (1974)

Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum.
по: Singhofer-Wowra, M, и др.
Опубликовано: (1986)

Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae.
по: Singhofer-Wowra, M, и др.
Опубликовано: (1986)

Relation between learning process and morphology of transport tube network in plasmodium of Physarum polycephalum
по: Emiri Yoneoka, и др.
Опубликовано: (2023-11-01)

Demonstration of different patterns of microtubule organization in Physarum polycephalum myxamoebae and plasmodia using immunofluorescence microscopy.
по: Havercroft, J, и др.
Опубликовано: (1983)

An Improved Physarum polycephalum Algorithm for the Shortest Path Problem
по: Xiaoge Zhang, и др.
Опубликовано: (2014-01-01)

Hydrophobic Barriers for Directing Physarum polycephalum Propulsion and Navigation
по: Taeha Lee, и др.
Опубликовано: (2023-10-01)

Sterol content of the Myxomycetes Physarum polycephalum and P. flavicomum
по: E Bullock, и др.
Опубликовано: (1976-11-01)

Incorporation of tubulin from an evolutionarily diverse source, Physarum polycephalum, into the microtubules of a mammalian cell.
по: Prescott, A, и др.
Опубликовано: (1989)

Synthesis of unsaturated fatty acids in the slime mold Physarum polycephalum and the zooflagellates Leishmania tarentolae, Trypanosoma lewisi, and Crithidia sp.: a comparative study
по: Edward D. Korn, и др.
Опубликовано: (1965-01-01)

Physarum Polycephalum Syllogistic L-Systems and Judaic Roots of Unconventional Computing
по: Schumann Andrew
Опубликовано: (2016-03-01)

Transcriptomic changes arising during light-induced sporulation in <it>Physarum polycephalum</it>
по: Glockner Gernot, и др.
Опубликовано: (2010-02-01)

Swarm Robot Exploration Strategy for Path Formation Tasks Inspired by Physarum polycephalum
по: Yandong Luo, и др.
Опубликовано: (2021-01-01)

Using an Artificial <i>Physarum polycephalum</i> Colony for Threshold Image Segmentation
по: Zhengying Cai, и др.
Опубликовано: (2023-11-01)

Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia
по: Tuyen T. H. Do, и др.
Опубликовано: (2021-03-01)

Vascular adaptation model from force balance: Physarum polycephalum as a case study
по: Sophie Marbach, и др.
Опубликовано: (2023-01-01)

A bio-inspired memory device based on interfacing Physarum polycephalum with an organic semiconductor
по: Agostino Romeo, и др.
Опубликовано: (2015-01-01)

Action spectrum in sporulation of slime-mold Physarum nudum Macbr.
по: L. Rakoczy
Опубликовано: (2015-01-01)

Semi-in vitro detection of Mg2+-dependent DNase that specifically digest mitochondrial nucleoids in the zygote of Physarum polycephalum
по: Naoki Urakawa, и др.
Опубликовано: (2022-02-01)

Making the mitotic spindle and axonal transport work in vitro.
по: Gull, K
Опубликовано: (1986)